遗传 ›› 2008, Vol. 30 ›› Issue (2): 190-194.

• 研究报告 • 上一篇    下一篇

RXRG基因遗传变异与双胎性状的关联分析

黄萌1; 许尚忠1,2; 昝林森1; 张路培2; 高雪2; 陈金宝2   

  1. 1. 西北农林科技大学动物科技学院, 杨凌 712100;
    2. 中国农业科学院北京畜牧兽医研究所, 北京 100094

  • 收稿日期:2007-09-05 修回日期:2007-11-15 出版日期:2008-02-10 发布日期:2008-02-10
  • 通讯作者: 许尚忠

Genetic variation in RXRG gene and its relationship with twinning trait in cattle

HUANG Meng1; XU Shang-Zhong1,2; ZAN Lin-Sen1; ZHANG Lu-Pei2; GAO Xue2; CHEN Jin-Bao2

  

  1. 1. College of Animal Science-technology, Northwest A &F University, Yangling 712100, China;
    2. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China
  • Received:2007-09-05 Revised:2007-11-15 Online:2008-02-10 Published:2008-02-10
  • Contact: XU Shang-Zhong

摘要:

以视黄素X受体基因g(retinoid X receptor-gamma, RXRG)作为牛双胎性状的候选基因, 运用测序法寻找牛RXRG基因SNPs, 筛查到一个新的多态位点A1941G, 该位点位于3′UTR。运用PCR-RFLP法验证并分析该位点在鲁西牛双胎群体和单胎群体及中国西门塔尔牛、安格斯牛和西蒙杂交牛单胎群体间的多态性, 结果表明, 在鲁西牛双胎和单胎群体中分布A、B两个等位基因,处于中度多态。经χ2适合性检验, 鲁西双胎牛群体在该位点未达到Hardy-Weinberg平衡状态(P < 0.05)。将鲁西牛群体的A1941G位点的基因型效应与双胎性状进行关联分析, 卡方独立性检测结果显示, 基因型分布在鲁西单、双胎牛群体上差异达到极显著水平(P < 0.01)。

关键词: 牛, RXRG基因, PCR-RFLP, 双胎性

Abstract:

Retinoic X receptor-gamma (RXRG) gene was studied as a candidate gene for the twinning trait of bovine. A new SNP A1941G was detected by sequencing at 3′UTR. Different genotypes were determined in Luxi monotocous cows, Luxi twinning cows, Chinese Simmental cows, Angus cows and Simmental×Mongolia cows by restriction fragment length polymorphism (RFLP). The value of polymorphism information content indicated that this was a moderate polymorphism in Luxi monotocous cows and Luxi twinning cows. The c2 test indicated that the polymorphic locus in Luxi twinning cows did not fit Hardy-Weinberg equilibrium (P<0.05). The c2 test of associational analysis between genotypic distribution and twinning or monotocous trait in Luxi cows showed that the difference was very significant (P<0.01).