关键词: 表达分析, 逆境, NAC转录因子, 米

Abstract: NAC transcription factors that are unique to plants are the new type transcription regulatory factors that have multiple biological functions. They play an important role in regulation of plant growth and development, hormone level and responses to various kinds stresses. One full-length cDNA encoding the NAC-like gene homologue was isolated from maize (Zea mays) by RT-PCR and named as ZmNAC1 (GenBank accession No., EU224278). Sequence analysis shows that it is 1029 bp long and contains a single open reading frame (ORF, 26~907 bp). The predicted ZmNAC1 protein has 293 amino acids with an estimated molecular mass of 32.3 KD and an isoelectric point of 8.65. RT-PCR analysis showed that the ZmNAC1expression was induced by low temperature, PEG, salt and ABA. These results suggest that ZmNAC1 may play important roles in abiotic resistance pathways. This is the first NAC-like gene reported in maize.