遗传 ›› 2010, Vol. 32 ›› Issue (10): 1077-1083.doi: 10.3724/SP.J.1005.2010.01077

• 研究报告 • 上一篇    下一篇

番茄可溶性固形物的动态QTL及相关性状

贾俊忠1, 田丽萍2, 薛琳3, 魏亦农4   

  1. 1. 石河子大学生命科学学院, 新疆石河子 832003; 
    2. 石河子大学药学院, 新疆石河子 832003; 
    3. 石河子蔬菜研究所, 新疆石河子 832003; 
    4. 新疆生产建设兵团绿洲生态重点实验室, 新疆石河子 832003
  • 收稿日期:2009-12-31 修回日期:2010-04-03 出版日期:2010-10-20 发布日期:2010-10-20
  • 通讯作者: 薛琳 E-mail:xuelin1806@163.com
  • 基金资助:

    十一五国家计划项目(编号:2006BAB01A7-3-09)资助

Dynamic QTL and correlated characters of tomato soluble solid content

JIA Jun-Zhong1, TIAN Li-Ping2, XUE Lin3, WEI Yi-Nong4   

  1. 1. College of Life Science, Shihezi University, Shihezi 832003, China;
     2. School of Pharmacy, Shihezi University, Shihezi 832003, China;
     3. Vegetable Research Institute of Shihezi, Shihezi 832003, China
    4. The key Oasis Eco-agriculture Laboratory of Xinjiang Production and Construction Group, Shihezi 832003, China
  • Received:2009-12-31 Revised:2010-04-03 Online:2010-10-20 Published:2010-10-20
  • Contact: XUE Lin E-mail:xuelin1806@163.com

摘要: 利用TD22×HT-1-1-1-1组合产生的206个F2:3家系进行番茄可溶性固形物及相关性状的动态QTL定位, 并对3个时期的可溶性固形物与果重、果型指数、可溶性糖、VC及有机酸进行相关性分析。结果表明在番茄果实发育的3个时期可溶性固形物的QTL位点存在差异, 绿熟期和红熟期分别检测到4个、8个QTL位点, 呈现动态变化, 两个时期同时检测到LEaat006 和Tomato|TC162363 两个标记, 对辅助选择育种有重要意义。研究发现3个时期可溶性固形物存在极显著差异, 不同时期可溶性固形物的主要相关性状不同。绿熟期与可溶性糖呈显著正相关, 与果重呈显著负相关; 黄熟期与可溶性糖和有机酸呈显著正相关; 红熟期与可溶性糖和有机酸呈显著正相关, 与果重呈显著负相关。在各性状相关性分析的基础上建立线性回归模型, 利用非参品种进行检验, 拟合度达到95%以上。

关键词: 线性回归模型, 数量性状基因座(QTL), 可溶性固形物, 相关性状, 番茄

Abstract: Two hundred and six F2:3 families from the cross between TD22 and HT-1-1-1-1 were used for dynamic QTL research of tomato soluble solid content and correlative traits, and correlation analysis of soluble solid content (SSC) with fruit weight (FW), fruit shape index (FSI), soluble sugar, vitamin C (VC), and organic acid at three different development stages. The results showed that there were differences in QTL loci for soluble solid content during the three stages of tomato fruit development. Four and eight QTLs were detected in green ripe stage and red ripe stage, respectively. These QTLs showed dynamic changes, and two markers LEaat006 and Tomato|TC162363 were detected in two stages, which might be useful in molecular-marker assisted selection (MAS). The result also showed that there was extremely significant difference in SSC at the three different stages, and its main correlative traits were different at different stages. Soluble solid content was positively correlated with soluble sugar, but negatively correlated with FW at green ripe stage; SSC was positively correlated with soluble sugar and organic acid at yellow ripe stage; SSC was positively correlated with soluble sugar and organic acid, but negatively correlated with fruit weight at red ripe stage. Based on correlation analysis of these traits, linear regression model was constructed. Non-tested varieties were used to test the fitness, and the result showed that it is well fitted, and the fitness is above 95%.

Key words: tomato, quantitative traits loci (QTL), soluble solid content (SSC), correlative traits, linear regression model