遗传 ›› 2011, Vol. 33 ›› Issue (12): 1327-1334.doi: 10.3724/SP.J.1005.2011.01327

• 综述 • 上一篇    下一篇

农杆菌介导转基因植物T-DNA的整合方式

杨琳, 付凤玲, 李晚忱   

  1. 四川农业大学玉米所, 成都 611130
  • 收稿日期:2011-03-30 修回日期:2011-07-25 出版日期:2011-12-20 发布日期:2011-12-25
  • 通讯作者: 李晚忱 E-mail:aumdyms@sicau.edu.cn
  • 基金资助:

    国家科技重大专项(编号:2009CB118400)和国家自然科学基金项目(编号:30971795, 31071433)资助

T-DNA integration patterns in transgenic plants mediated by Agrobacterium tumefaciens

YANG Lin, FU Feng-Ling, LI Wan-Chen   

  1. Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, China
  • Received:2011-03-30 Revised:2011-07-25 Online:2011-12-20 Published:2011-12-25

摘要: 农杆菌介导的遗传转化已被广泛应用于植物转基因研究。作为外源基因的载体, 农杆菌T-DNA片段在植物基因组中的整合方式, 不仅影响外源基因的整合效率及稳定性, 还会影响外源基因的表达特性。文章就农杆菌介导的T-DNA整合的两种主要模式、规律及相关研究手段进行综述, 为农杆菌介导的转基因及T-DNA插入突变等相关研究提供借鉴。

关键词: 农杆菌, T-DNA, 侧翼序列, 整合, 转基因

Abstract: The genetic transformation mediated by Agrobacterium tumefaciens has been widely applied to research of transgenic plants. As the vector of the exotic genes, the integration patterns of T-DNA fragments affects not only transformation efficiency and stability, but also expression properties of the transgenes. This review summaries the two major patterns and the rules of T-DNA integration in Agrobacterim-mediated transformation, rules of T-DNA me-diated by Agrobacterium tumefaciens, as well as research tools for flanking sequence amplification. It is attempted to provide references for researches on transformation and T-DNA integration mutation mediated by Agrobacterium tumefaciens.

Key words: Agrobacterium tumefaciens, transfer DNA, flanking sequence, integration, transgene