遗传 ›› 2005, Vol. 27 ›› Issue (3): 403-409.

• 研究报告 • 上一篇    下一篇

远缘杂交油菜核不育系的创建及其细胞学和形态学研究

蔺兴武;吴建国;石春海   

  1. 浙江大学农业与生物技术学院农学系,杭州 310029
  • 收稿日期:2004-05-06 修回日期:2004-06-21 出版日期:2005-06-10 发布日期:2005-06-10
  • 通讯作者: 石春海

Establishment of Genic Male Sterile Lines of Brassica Napus by Wide Cross and Their Cytology and Morphology

LIN Xing-Wu;WU Jian-Guo;SHI Chun-Hai   

  1. Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University,
  • Received:2004-05-06 Revised:2004-06-21 Online:2005-06-10 Published:2005-06-10
  • Contact: SHI Chun-Hai

摘要: 在甘蓝型油菜与诸葛菜以及芥菜型油菜与诸葛菜属间杂交后代中分别发现1个和3个不育材料,经杂交和多代近交育成了相应的甘蓝型油菜不育系。通过对核不育系体细胞鉴定表明,所有新发现的不育系染色体数为38,均已恢复到甘蓝型油菜。这些不育系绝大部分花粉母细胞(PMC)在中期Ⅰ、后期Ⅰ和后期Ⅱ 3个时期染色体行为表现正常,但不同时期的PMC均会出现一定比例的异常现象,主要表现为染色体落后或染色体桥等。这些不育系属于单核败育型,不育株与可育株的花器形态差异明显,不育系还存在不同程度的死蕾等特点。通过对花器生长过程的研究,发现不育株雌蕊生长随雄蕊败育进程逐渐加快,而可育株雌蕊生长则存在两个生长缓慢阶段。此外,文章还讨论了这些不育系的应用前景。

关键词: 远缘杂交, 甘蓝型油菜, 细胞核雄性不育, 细胞学, 形态学

Abstract: One and three male sterile plants were found in the progenies of the intergeneric hybrids by crossing Brassica napus and Brassica juncea to Orychophragmus violaceus, respectively. After interspecific cross and full-sib crosses, four genic male sterile lines were obtained. The results of cytological observations indicated that somatic chromosome number was 38 in all types of sterile lines, which were considered as the type of B. napus. Most of pollen mother cells (PMC) in all sterile plants were observed normally for chromosome behaviour. But abnormal phenomena of chromosome, which present for chromosome lagging and bridge, were still found at each stage of meiosis in these sterile lines. These lines belonged to complete sterile type. There were distinct differences between sterile plants and normal plants in the flower morphology. Bud death at different degrees was found on anthotaxies in sterile plants. Studies on the flower growth showed that the growths of the pistils in sterile plants were gradually accelerated along with pollen sterility. At the same time there were two slowly growth stages of the pistils growth in normal plants. The prospects of these sterile lines were discussed in this paper.

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