真菌类过氧化物酶体增殖因子PEX11基因家族生物信息学研究

doi:10.3724/SP.J.1005.2012.00635

遗传 ›› 2012, Vol. 34 ›› Issue (5): 635-646.doi: 10.3724/SP.J.1005.2012.00635

真菌类过氧化物酶体增殖因子PEX11基因家族生物信息学研究

张昕^1,2, 姜华², 王艳丽², 张震², 毛雪琴², 柴荣耀², 邱海萍², 杜新法², 王教瑜²,孙国昌²

1. 杭州师范大学生命与环境科学学院, 杭州310036 2. 浙江省植物有害生物防控重点实验室-省部共建国家重点实验室培育基地, 浙江省农业科学院植物保护与微生物研究所, 杭州 310021

收稿日期:2011-08-18 修回日期:2011-10-24 出版日期:2012-05-20 发布日期:2012-05-25
通讯作者: 王教瑜 E-mail:jiaoyuwang1@gmail.com
基金资助:
国家自然科学基金项目(编号：30900933; 30970082; 31170136)资助

Bioinformaticresearch of the family of PEX11, peroxisomeproliferous factor in fungus

ZHANG Xin^1,2, JIANG Hua², WANG Yan-Li², ZHANG Zhen², MAO Xue-Qin², CHAI Rong-Yao², QIU Hai-Ping², DU Xin-Fa², WANG Jiao-Yu², SUN Guo-Chang²

1. College of Life and Environment Sciences, Hangzhou Normal University, Hangzhou 310036, China 2. State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection Microbiology, Zhejiang Academy of Agricultural sciences, Hangzhou 310021, China

Received:2011-08-18 Revised:2011-10-24 Online:2012-05-20 Published:2012-05-25

摘要/Abstract

摘要： PEX11基因家族成员是参与过氧化物酶体增殖调控的关键因子, 文章利用生物信息学方法对26种代表性真菌的PEX11基因家族成员进行了检索和进化分析。研究发现：(1)26种真菌中共有66个可能的PEX11p。酵母类真菌有1个或2个PEX11p, 而大多数丝状真菌中包含2到3个, 其中子囊菌中PEX11p的个数偏多, 个别种类达到5个; (2)真菌PEX11p可分为3类, 大多数真菌含有类型Ⅰ和类型Ⅲ的PEX11p, 类型Ⅱ是盘菌亚门真菌所特有的, 可能与类型Ⅰ和类型Ⅲ在功能上有冗余; (3)通过MEME分析, 发现PEX11p含有多个保守区域, 其中C末端的Motif8具有很高的保守性, 推测可能对PEX11p发挥功能具有重要作用。文章对进一步研究真菌PEX11p的进化与功能以及过氧化物酶体的增殖具有重要意义。

关键词: 真菌, 过氧化物酶体, PEX11基因家族, 生物信息学, 进化分析

Abstract: The family members of PEX11 are key factors involved in regulation of peroxisome proliferation. Sixty-six PEX11p candidates of PEX11 gene family from26 representative fungal species were obtainedand analyzed by bioinformatic strategies. In most filamentous fungi, 2 or 3 potential PEX11ps were found, in contrast with 1 or 2 in yeast species. Com-pared with other fungal species, the Ascomycetes tend to have more PEX11ps, and even 5 in several individuals. The data of phylogenetic analysis and protein structure indicated that all of the PEX11ps were divided into 3groups: I, II, and III. The members of group I and groupIIIexisted in most species, while those in group II were found only in Pezizomycotina. By MEME analysis, 5-6 conserved motifs were found in each PEX11ps. Among them,motif 8 in C-terminal had the most conservation, indicating that this motif probably plays a key role in maintaining the proper functionof PEX11p.

Key words: PEX11 gene family, bioinformatics, phylogenetic analysis, fungus, peroxisome

张昕，姜华，王艳丽，张震，毛雪琴，柴荣耀，邱海萍，杜新法，王教瑜，孙国昌. 真菌类过氧化物酶体增殖因子PEX11基因家族生物信息学研究[J]. 遗传, 2012, 34(5): 635-646.

ZHANG Xin, JIANG Hua, WANG Yan-Li, ZHANG Zhen, MAO Xue-Qin, CHAI Rong-Yao, QIU Hai-Ping, DU Xin-Fa, WA Jiao-Yu, SUN Guo-Chang. Bioinformaticresearch of the family of PEX11, peroxisomeproliferous factor in fungus[J]. HEREDITAS, 2012, 34(5): 635-646.

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真菌类过氧化物酶体增殖因子PEX11基因家族生物信息学研究

Bioinformaticresearch of the family of PEX11, peroxisomeproliferous factor in fungus

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