遗传 ›› 1999, Vol. 21 ›› Issue (3): 9-714.

• 论文 • 上一篇    下一篇

水稻抗白叶枯病基因Xa-4的PCR标记研究

马伯军12;王文明1;赵彬1;周永力3;朱立煌1;翟文学1 MA Bo-Jun1;2);WANG Wen-Ming1);ZHAO Bin1);ZHOU Yong-Li3);ZHU Li-Huang1);ZHAI Wen-Xue1)   

  1. 1.中国科学院遗传研究所,北京100101 2.中国农业科学院作物育种栽培研究所,北京100081      1.Institute of Genetics,The Chinese Academy of Sciences,Beijing100101 2.Biology Department of Zhejiang Normal University,Jinhua321004 3.Institute of Crop Breeding and Cultivation,The Chinese Academy of Agricultural Sciences,Beijing100081
  • 收稿日期:1900-01-01 出版日期:1999-06-10 发布日期:1999-06-10

Studies of PCR Marker for the Rice Bacterial Blight Resistance Gene Xa-4

  • Received:1900-01-01 Online:1999-06-10 Published:1999-06-10

摘要: 根据与水稻抗白叶枯病基因Xa-4紧密连锁的分子标记M55的序列设计引物,通过对国际水稻研究所育成的抗白叶枯病近等基因系和基因累加系的叶片DNA、半粒种子提取物及Xa-4基因的杂合体DNA的PCR特异扩增,初步建立了Xa-4的PCR标记体系。进而用该标记体系对我国籼型杂交水稻常用的亲本材料进行分析,揭示出了Xa-4在这些材料中的分布情况。
Abstract Based on the sequence of a DNA marker tightly linked to the rice bacterial blight(BB) resistance gene Xa-4, two primers were designated and synthesized to develop a PCR marker for the gene. Specific amplified polymorphism analysis was carried out with these primers on a set of BB resistance isogenic lines and pyramided lines developed by IRRI. Two PCR bands were revealed corresponding to lines with dominant Xa-4 and those with the recessive allele, respectively, regardless the lines pyramided with other resistance genes. A hybrid with heterozygous Xa-4 produced both of the two allele PCR pattern. Then, the PCR marker was used to survey a range of hybrid rice germplasm. The results of the germplasm survey will be useful in hybrid rice breeding programs aimed at exploiting Xa-4.
  
  

关键词: PCR标记 Key words Rice, Bacterial blight disease, Xa-4基因, 白叶枯病, 水稻