遗传 ›› 2000, Vol. 22 ›› Issue (5): 303-304.

• 论文 • 上一篇    下一篇

利用CLSM检测GFP基因在家蚕的瞬时表达

陈智毅;李清兵;陈列辉;廖森泰;李宝瑜;廖琼香 CHEN Zhi-yi;LI Qing-bing;CHEN Lie-hui;LIAO Seng-tai;LI Bao-yu;LIAO Qiong-xiang   

  1. 广东省农业科学院蚕业研究所,广东五山 510640 Sericultural Institute of Guangdong,Guangzhou 510640,China
  • 收稿日期:1900-01-01 出版日期:2000-10-10 发布日期:2000-10-10

Expression of GFP Gene in Silkworm Examined by CLSM

  • Received:1900-01-01 Online:2000-10-10 Published:2000-10-10

摘要: 将绿色荧光蛋白(GFP)基因导入农蚕蛹(G0)的睾丸,利用激光共聚焦扫描显微镜(CLSM)系统检测,得到GFP基因在家蚕刚孵出未进食的幼虫(蚁蚕)(G1)瞬时表达的荧光图像。
Abstract:Plasmid DNA containing the GFP(Green Fluorescent Protein)reporter gene was injected into the testis of silkworm(Bombyx mori L.)pupa(G0),we observe the fluorescence imaging of expression of GFP gene in silkworm newly-hatched larvae(G1)examined by Confocal Laser Scanning Microscope.

关键词: 转基因, 绿色荧光蛋白, 共聚焦扫描显微镜
Key words,
Bombyx mori L., 家蚕