遗传 ›› 1998, Vol. 20 ›› Issue (3): 23-26.

• 论文 • 上一篇    下一篇

基因枪转移Bar基因获得抗除草剂(Basta)的糜子蔗克隆再生植株

母秋华1; 原亚萍1; 贾玉峰1; 王金余1; 田文忠2; 何锶洁2; 李良材2 MU Qiu-hua1;YUAN Ya-ping1;JIA Yu-feng1;WANG Jin-yu1;TIAN Wen-zhong2;HE Si-jie2;LI Liang-cai2   

  1. 1.解放军农牧大学植物细胞工程研究室, 长春 130062 2.中国科学院遗传研究所,北京 100101 1.Plant Cell Engineering Lab.,University of Agricultural and Animal Science of PLA,Changchun 130062 2.Institute of Genetics,Chinese Academy of Sciences,Beijing 100101
  • 收稿日期:1900-01-01 出版日期:1998-06-10 发布日期:1998-06-10

Regenerated Plants Resistanted to Basta were Obtained ThroughIntroduction of Bar Genes into [(Sorghum*Sugarcane)*Mi lletSorghum]Using Particle Bombardment

  • Received:1900-01-01 Online:1998-06-10 Published:1998-06-10

摘要: 用糜子蔗(sorghum×sugarcane)×millet sorghum茎尖愈伤组织为受体,通过基因枪轰击将Bar基因转入糜子蔗细胞;用除草剂Basta含量分别为1、2.5、5、10mg/L的8114诱导与分化培养基上选择抗性愈伤组织;在Bast a含量为1与1.5mg/L筛选培养基上,选择出3个快速增殖并大量再生绿苗的体细胞无性系(Clone),如BarC1、BarC2、BarC3;BarC1、C2再生植株用Basta含量为1、2.5-5mg/L的溶液进行抗性检测,证明这些小植株具有极强的抗性。
Abstract:The Bar genes were introduced into〔(sorghum×sugarcane) ×millet sorghum〕callus cells using particle bombardment.The callus were cultured on the 8114 media with Basta,the Bastas concentration is 1,2.5 and 5,10mg/L.3 Clones with regenerated capacity were screened out on the media with Basta 1,2.5mg/L,for example BarC1,BarC2,BarC3.The regenerated plant from BarC1、BarC2 were cultured on the solution with Basta 1.5,2.5,and 5mg/L,high resistance were obtained in these regenerated plants.

关键词: Bar genes transformation, (Sorhum×Sugarcane) ×millet sorghum, 植株再生
Key words,
Bar基因转移, 糜子蔗, 广亲和基因, 不育性, 遗传分析, 水稻(Oryza sativa L.), 籼粳杂交

Key words: Sterility, Oryza sativa, Wide compatibility gene, indica japonica cross, Genetic analysis