遗传 ›› 2017, Vol. 39 ›› Issue (8): 737-752.doi: 10.16288/j.yczz.16-435

• 综述 • 上一篇    下一篇

陆地棉GST基因家族全基因组分析

许磊(),陈文,司国阳,黄艺园,林毅,蔡永萍,高俊山()   

  1. 安徽农业大学生命科学学院,合肥 230036
  • 收稿日期:2017-01-03 修回日期:2017-05-10 出版日期:2017-08-20 发布日期:2017-12-25
  • 作者简介:许磊,硕士研究生,专业方向：细胞生物学。E-mail: 437185342@qq.com|高俊山,教授,硕士生导师,研究方向：植物分子遗传学。E-mail: gaojsh@ahau.edu.cn
  • 基金资助:
    国家自然科学基金项目(31672497);安徽省“115”产业创新团队项目([2011]2)

Genome-wide analysis of the GST gene family in Gossypium hirsutum L.

Lei Xu(),Wen Chen,Guoyang Si,Yiyuan Huang,Yi Lin,Yongping Cai,Junshan Gao()   

  1. College of life science, Anhui Agricultural University, Hefei 230036, China
  • Received:2017-01-03 Revised:2017-05-10 Online:2017-08-20 Published:2017-12-25
  • Supported by:
    the National Natural Science Foundation of China(31672497);Anhui Province “115” Industrial Innovation team(([2011]2)

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摘要：

谷胱甘肽转移酶(glutathione-S-transferase, GST)是一种普遍存在的具有多功能的超家族蛋白,在植物初次生代谢、逆境胁迫、胞间信号传递等方面具有重要作用;同时,作为配体其在植物激素代谢以及物质转运方面也发挥作用。为了解析陆地棉(Gossypium hirsutum L.) GST基因家族的信息,本研究对该基因家族成员的种类、进化关系、物理定位、基因结构和保守基序以及表达模式进行了分析。结果显示,在陆地棉全基因组中共含有70个GST基因,进化树和基因结构分析将该家族分为U族、F族、T族、Z族、EF1Bγ族和TCHQD族。基因定位分析发现,除了AD/At2、AD/At4、AD/At5、AD/Dt5、AD/Dt10号染色体上没有GST基因外,其他染色体上都有GST基因,并且在AD/At9、AD/Dt7、AD/Dt12、AD/Dt13这4条染色体上出现基因簇。对F族(Phi类) 9个GST基因进行荧光定量分析,结果表明,除GhGSTF1可能为假基因外,GhGSTF2~9等8个基因在陆地棉根、茎、叶以及各个发育时期的纤维中均有表达;结合生物信息学分析,推测GhGSTF8可能参与原花青素/花青素的转运和积累;GhGSTF4、GhGSTF6和GhGSTF9可能在调节陆地棉的生长和胁迫反应中起作用,而GhGSTF2、GhGSTF3、GhGSTF5和GhGSTF7的功能还有待进一步研究。本研究为陆地棉GST基因家族的分子进化及功能研究提供了理论依据。

关键词: 陆地棉, 谷胱甘肽转移酶, 全基因组分析, 进化

Abstract:

Glutathione-S-transferase (GST) is a ubiquitous multi-functional protein superfamily that plays important roles in plant primary and secondary metabolism, stress and intercellular signal transduction. Concomitantly, it also functions as a ligand in the metabolism of plant hormones and substance transport. In order to understand the GST gene family in upland cotton (Gossypium hirsutum L.), herein we analyzed the species, evolutionary relationship, physical location, gene structure, conserved motifs and expression patterns. We identified 70 GST genes in the whole genome of upland cotton, and divided them into U, F, T, Z, EF1Bγ and TCHQD groups by phylogenetic tree and gene structure analyses. The gene mapping analysis indicated that the GST genes were on every chromosome except chromosome AD/At2, AD/At4, AD/At5, AD/Dt5 and AD/Dt10. Moreover, the GST gene cluster appeared on four chromosomes (AD/At9, AD/Dt7, AD/Dt12 and AD/Dt13). qRT-PCR assays showed that eight genes (GhGSTF2-9) were expressed in the root, stem, leave and fiber of different developmental stages while GhGSTF1 might be a pseudogene. Combining qRT-PCR and bioinformatic analysis, we speculated that GhGSTF8 might be involved in the transport and accumulation of proanthocyanidins/anthocyanins; GhGSTF4, 6 and 9 might play roles in regulating the growth and stress response of upland cotton; the function of GhGSTF2, 3, 5 and 7 remains to be further investigated. Our work provides a theoretical basis for further studies on the molecular evolution and function of the GST gene family in upland cotton.

Key words: Gossypium hirsutum L., glutathione-S-transferase (GST), genome-wide analysis, evolution