遗传 ›› 2003, Vol. 25 ›› Issue (3): 307-310.

• 论文 • 上一篇    下一篇

利用基因芯片检测转基因作物

黄迎春;孙春昀;冯红;胡晓东;尹海滨 HUANG Ying-Chun;SUN Chun-Yun;FENG Hong;HU Xiao-Dong;YIN Hai-Bin   

  1. 成都百奥生物信息科技有限公司,成都东胜街8号 610015 Chengdu Bioinformation Technique Incorporation,Dongsheng Rood No.8,Chengdu 610015,China
  • 收稿日期:1900-01-01 出版日期:2003-06-10 发布日期:2003-06-10

Detection of Transgenic Crop with Gene Chip

  • Received:1900-01-01 Online:2003-06-10 Published:2003-06-10

摘要: 选用常用的两种报告基因、两种抗性基因、两种启动子序列和两种终止子序列为探针,将其PCR扩增产物用MicroGrid Ⅱ型全自动点样仪按矩阵排列点样于包埋有氨基的载玻片上,制备成转基因作物检测型基因芯片。利用该芯片对4种转基因水稻、木瓜、大豆、玉米进行检测,结果表明,该芯片能对转基因作物做出快速、准确的检测。
Abstract:Some selected available sequences of reporter genes,resistant genes,promoters and terminators are amplified by PCR for the probes of transgenic crop detection gene chip.These probes are arrayed at definite density and printed on the surface of amino-slides by bioRobot MicroGrid Ⅱ.Results showed that gene chip worked quickly and correctly,when transgenic rice,pawpaw,maize and soybean were applied.

关键词: transgenic crop, 检测
Key words,
gene chip, 转基因作物, 基因芯片