遗传 ›› 2003, Vol. 25 ›› Issue (4): 414-418.

• 论文 • 上一篇    下一篇

新疆三种雅罗鱼属鱼类mtDNA D-loop多态性及起源分化分析

胡文革1;王金富2;盛金良1;段子渊3;马润林3 HU Wen-Ge1;WANG Jin-Fu2;SHENG Jin-Liang1;DUAN Zi-Yuan3;MA Run-Lin3   

  1. 1.石河子大学生物工程学院,新疆 石河子 832003; 2.石河子大学分子生物学实验室,石河子832003; 3.中国科学院遗传与发育生物学研究所,北京100101 1.Bio-engineer College,Shihezi Unive rsity,Shihezi,Xinjiang 832003,China; 2.Molecular Biological Laboratory,Shihezi University,Shihezi,Xinjiang 832003,China; 3.Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing100101,China
  • 收稿日期:1900-01-01 出版日期:2003-08-10 发布日期:2003-08-10

Polymorphism and Original Analysis of mtDNA D-loop of Three Leuciscus Species in Xinjiang

  • Received:1900-01-01 Online:2003-08-10 Published:2003-08-10

摘要: 用PCR-RFLP技术,对新疆分布的准噶尔雅罗鱼、贝加尔雅罗鱼和高体雅罗鱼的mtDNA D-loop高变区约827bp进行了扩增,用ScaI、HinfI、AluI、DdeI 4种限制性内切核酸酶对56个样本的扩增产物酶切和RFLP分析,共检测到6种单倍型。准噶尔雅罗鱼存在2种单倍型:BDAA和BDBA;贝加尔雅罗鱼存在3种:AAAA、ABAA和ACAA;高体雅罗鱼只有1种。初步认为,准噶尔雅罗鱼、贝加尔雅罗鱼存在较丰富的群体内变异。用6种单倍型及净遗传距离绘制的UPGMA分子系统树,提示准噶尔雅罗鱼有可能是原始种,贝加尔雅罗鱼和高体雅罗鱼是由准噶尔雅罗鱼进化而来。
Abstract:PCR-RFLP technique was employed to amplify about 827bp of mtDNA D-loop hypervariable region of Leuciscus baicalensis,L.merzbacheri and L.idus in Xinjiang.The PCR products of 56 samples with four restriction enzymes were digested:ScaI,HinfI,AluI,DdeI,and RFLP analysis was done then.The study indicates that all of L. species and populations have six haplotypes,L.merzbacheri has two haplotypes:BDAA,BDBA;L.baicalensis has three:AAAA,ABAA,ACAA;L.idus has one:CAAA.It is primarily considered that L.merzbacheri and L.baicalensis have more intra-population mutations.The UPGMA trees with 6 haplotypes and net genetics distance pointed out that L.merzbacheri might be original species,L.baicalensis and L.idus are evolved from L.merzbacheri.

关键词: 汉族, 脐带血, 姓氏, DNA库, 籍贯, mtDNA D-loop, 新疆三种雅罗鱼, PCR-RFLP, 多态性, 进化
Key words

Key words: DNA bank, Han nation, surname, cord blood, native place