IGHMBP2过表达促进食管鳞癌细胞的侵袭和迁移

doi:10.16288/j.yczz.14-371

[an error occurred while processing this directive]

HEREDITAS(Beijing) ›› 2015, Vol. 37 ›› Issue (4): 360-366.doi: 10.16288/j.yczz.14-371

• Research Articles • Previous Articles Next Articles

IGHMBP2 overexpression promotes cell migration and invasion in esophageal squamous carcinoma

Chunli Wang¹,Jiajie Hao²,Lifei Wu³,Beiqing Pan²,Xin Xu²,Yan Cai²,Mingrong Wang²,Xuemei Jia¹

1. Department of Histology and Embryology, Anhui Medical University, Hefei 230032, China;
2. State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, China;
3. Department of Gastroenterology, Anqing Municipal Hospital of Anhui Medical University, Anqing 246000, China

Received:2014-10-29 Online:2015-04-20 Published:2015-03-06

Abstract

Abstract: Immunoglobulin mu binding protein 2 (IGHMBP2) is located in 11q13.2, which is frequently amplified in esophageal squamous cell carcinoma (ESCC). IGHMBP2 encodes a helicase involved in DNA replication and repair. IGHMBP2 protein also regulates gene transcription. The present study aims to explore the amplification of IGHMBP2 and its potential role in ESCC. A further analysis of our previously reported array-CGH data showed that IGHMBP2 was amplified in 28.9% of primary ESCC tumors. Fluorescence in situ hybridization (FISH) and Western blot showed that IGHMBP2 was amplified and overexpressed in KYSE30, KYSE180, KYSE510 and KYSE150 esophageal cancer cell lines. Transwell assays demonstrated that knockdown of IGHMBP2 in KYSE30 and KYSE150 inhibited cell invasion and migration, and increased the expression levels of E-cadherin. When rescue plasmids expressing IGHMBP2 were introduced, the abilities of cell invasion and migration were restored. These data suggest that IGHMBP2 overexpression may promote invasion and migration of ESCC cells through down-regulation of E-cadherin.

Key words: IGHMBP2, esophageal squamous cell carcinoma, amplification, invasion, migration

Chunli Wang,Jiajie Hao,Lifei Wu,Beiqing Pan,Xin Xu,Yan Cai,Mingrong Wang,Xuemei Jia. IGHMBP2 overexpression promotes cell migration and invasion in esophageal squamous carcinoma[J]. HEREDITAS(Beijing), 2015, 37(4): 360-366.

References

[1] van Hagen P, Hulshof MCCM, van Lanschot JJB, Steyerberg EW, van Berge Henegouwen MI, Wijnhoven BPL, Richel DJ, Nieuwenhuijzen GAP, Hospers GAP, Bonenkamp JJ, Cuesta MA, Blaisse RJB, Busch ORC, ten Kate FJW, Creemers GJ, Punt CJA, Plukker JTM, Verheul HMW, Spillenaar Bilgen EJ, van Dekken H, van der Sangen MJC, Rozema T, Biermann K, Beukema JC, Piet AHM, van Rij CM, Reinders JG, Tilanus HW, van der Gaast A, CROSS Group. Preoperative chemoradiotherapy for esophageal or junctional cancer. N Engl J Med , 2012, 366(22): 2074-2084.
[2] Siegel R, Naishadham D, Jemal A. Cancer statistics, 2013. CA: A Cancer J Clin , 2013, 63(1): 11-30.
[3] Miyawaki Y, Kawachi H, Ooi A, Eishi Y, Kawano T, Inazawa J, Imoto I. Genomic copy-number alterations of MYC and FHIT genes are associated with survival in esophageal squamous-cell carcinoma. Cancer Sci , 2012, 103(8): 1558-1566.
[4] Kornegoor R, Moelans CB, Verschuur-Maes AHJ, Hogenes MCH, de Bruin PC, Oudejans JJ, Marchionni L, van Diest PJ. Oncogene amplification in male breast cancer: analysis by multiplex ligation-dependent probe amplification. Breast Cancer Res Treat , 2012, 135(1): 49-58.
[5] Brown JR, Hanna M, Tesar B, Werner L, Pochet N, Asara JM, Wang YE, Dal Cin P, Fernandes SM, Thompson C, MacConaill L, Wu CJ, Van de Peer Y, Correll M, Regev A, Neuberg D, Freedman AS. Integrative genomic analysis implicates gain of PIK3CA at 3q26 and MYC at 8q24 in chronic lymphocytic leukemia. Clin Cancer Res , 2012, 18(14): 3791-3802.
[6] Xu FP, Xie D, Wen JM, Wu HX, Liu YD, Bi J, Lü ZL, Zeng YX, Guan XY. SRC-3/AIB1 protein and gene amplification levels in human esophageal squamous cell carcinomas. Cancer Lett , 2007, 245(1-2): 69-74.
[7] Solyanik GI. Multifactorial nature of tumor drug resistance. Exp Oncol , 2010, 32(3): 181-185.
[8] Harvey H, Piskareva O, Creevey L, Alcock LC, Buckley PG, O'Sullivan MJ, Segura MF, Gallego S, Stallings RL, Bray IM. Modulation of chemotherapeutic drug resistance in neuroblastoma SK-N-AS cells by the neural apoptosis inhibitory protein and miR-520f. Int J Cancer , 2014, doi:10.1002/ijc.29144.
[9] Ong CA, Shannon NB, Ross-Innes CS, O'Donovan M, Rueda OM, Hu DE, Kettunen MI, Walker CE, Noorani A, Hardwick RH, Caldas C, Brindle K, Fitzgerald RC. Amplification of TRIM44: pairing a prognostic target with potential therapeutic strategy. J Natl Cancer Inst , 2014, 106(5), doi:10.1093/jnci/dju050.
[10] Ying JM, Shan L, Li JS, Zhong L, Xue LY, Zhao H, Li LL, Langford C, Guo L, Qiu T, Lu N, Tao Q. Genome-wide screening for genetic alterations in esophageal cancer by aCGH identifies 11q13 amplification oncogenes associated with nodal metastasis. PLoS One , 2012, 7(6): e39797.
[11] Togashi Y, Arao T, Kato H, Matsumoto K, Terashima M, Hayashi H, de Velasco MA, Fujita Y, Kimura H, Yasuda T, Shiozaki H, Nishio K. Frequent amplification of ORAOV1 gene in esophageal squamous cell cancer promotes an aggressive phenotype via proline metabolism and ROS production. Oncotarget , 2014, 5(10): 2962-2973.
[12] Luo ML, Shen XM, Zhang Y, Wei F, Xu X, Cai Y, Zhang X, Sun YT, Zhan QM, Wu M, Wang MR. Amplification and overexpression of CTTN ( EMS1 ) contribute to the metastasis of esophageal squamous cell carcinoma by promoting cell migration and anoikis resistance. Cancer Res , 2006, 66(24): 11690-11699.
[13] Shi ZZ, Shang L, Jiang YY, Hao JJ, Zhang Y, Zhang TT, Lin DC, Liu SG, Wang BS, Gong T, Zhan QM, Wang MR. Consistent and differential genetic aberrations between esophageal dysplasia and squamous cell carcinoma detected by array comparative genomic hybridization. Clin Cancer Res , 2013, 19(21): 5867-5878.
[14] Hu N, Wang CY, Ng D, Clifford R, Yang HH, Tang ZZ, Wang QH, Han XY, Giffen C, Goldstein AM, Taylor PR, Lee MP. Genomic characterization of esophageal squamous cell carcinoma from a High-Risk population in China. Cancer Res ,

Metrics

Comments

www.chinagene.cn
备案号：京ICP备09063187号

[1]	Danni Liu, Haiping Wu, Guohua Zhou. Research progress of visual detection in rapid on-site detection of pathogen nucleic acid [J]. Hereditas(Beijing), 2023, 45(4): 306-323.
[2]	Jing’ao Lu, Chunyan Huang, Zhiyin Lin, Zheng Tang, Ning Ma, Zhibin Huang. The role of the cd99l2 gene on leukocyte interstitial migration in zebrafish [J]. Hereditas(Beijing), 2022, 44(9): 798-809.
[3]	Zhilu Fan, Liyan Yang, Na Zhang, Dan Feng, Jing Guo, Chen Chang, Qing Yuan, Yan Cai, Yu Zhang, Wenqiang Wei, Mingrong Wang, Jiajie Hao. NEFL promotes invasion and migration of esophageal squamous carcinoma cells via the EGFR/AKT/S6 pathway [J]. Hereditas(Beijing), 2022, 44(4): 322-334.
[4]	Cuiling Wang, Xinyi Liu, Yahui Wang, Zheng Zhang, Zhidong Wang, Gangqiao Zhou. MCM2 promotes the proliferation, migration and invasion of cholangiocarcinoma cells by reducing the p53 signaling pathway [J]. Hereditas(Beijing), 2022, 44(3): 230-244.
[5]	Di Wang, Liyan Yang, Zou Liu, Jing Yu, Minjie Zhang, Yu Zhang, Yan Cai, Xin Xu, Jiajie Hao, Mingrong Wang. PAI-1 overexpression promotes invasion and migration of esophageal squamous carcinoma cells [J]. Hereditas(Beijing), 2020, 42(3): 287-295.
[6]	Xiangpeng He,Bingjie Zou,Xiemin Qi,Shan Chen,Yan Lu,Qing Huang,Guohua Zhou. Methods of isothermal nucleic acid amplification-based microfluidic chips for pathogen microorganism detection [J]. Hereditas(Beijing), 2019, 41(7): 611-624.
[7]	Fang Li,Qingyun Huang,Sijia Liu,Zhongxin Guo,Xinxin Xiong,Lin Gui,Huijuan Shu,Shaoming Huang,Guohe Tan,Yuanyuan Liu. The role of Bmal1 in neuronal radial migration and axonal projection of the embryonic mouse cerebral cortex [J]. Hereditas(Beijing), 2019, 41(6): 524-533.
[8]	Yaxin Yao,Yongfu La,Ran Di,Qiuyue Liu,Wenping Hu,Xiangyu Wang,Mingxing Chu. Comparison of different single cell whole genome amplification methods and MALBAC applications in assisted reproduction [J]. Hereditas(Beijing), 2018, 40(8): 620-631.
[9]	Xiulian Shen, Yichao Lu, Zhilian Jia, Qiang Wu. N-WASP regulates cortical neuron migration through its polyPro and VCA domains [J]. Hereditas(Beijing), 2018, 40(5): 390-401.
[10]	Junyu Zhang,Shan Lv,Huimin Niu,Anmin Lei. Research progress on the asymmetric division in mammalian oocytes [J]. Hereditas(Beijing), 2018, 40(4): 279-291.
[11]	Hui Liang, Guojie Chen, Yan Yu, Likuan Xiong. Co-amplification at lower denaturation temperature-PCR: methodology and applications [J]. Hereditas(Beijing), 2018, 40(3): 227-236.
[12]	Yan Zhou, Chaojie Wang, Chunchao Zhu, Jiangrong Chen, Ming Cheng, Yuliang Deng, Yan Guo, . Single-cell gene variation analysis method for single gland [J]. Hereditas(Beijing), 2017, 39(8): 753-762.
[13]	lu Yang, Zhuangzhi Zou, , Beibei Mao, . The roles of YAP T425A mutation in the regulation of YAP activity [J]. Hereditas(Beijing), 2017, 39(7): 675-682.
[14]	Wenhui Lin, Bingjie Zou, Qinxin Song, Guohua Zhou. Progress in multiplex loop-mediated isothermal amplification technology [J]. HEREDITAS(Beijing), 2015, 37(9): 899-910.
[15]	Yingying Li,Zhiguang Liu,Li Wang,Yuanyuan Yuan,Ping Liu,Linsong Wang. Serum miRNAs as new biomarkers for esophageal squamous cell carcinoma [J]. HEREDITAS(Beijing), 2015, 37(4): 315-320.

IGHMBP2 overexpression promotes cell migration and invasion in esophageal squamous carcinoma

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments