雷蒙德氏棉和亚洲棉基因组数据中LPAAT基因家族的发掘及其同源基因在陆地棉材料中的表达分析

doi:10.16288/j.yczz.15-010

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HEREDITAS(Beijing) ›› 2015, Vol. 37 ›› Issue (7): 692-701.doi: 10.16288/j.yczz.15-010

• Research Articles • Previous Articles Next Articles

Genome-wide analysis of the LPAAT gene family in Gossypium raimondii and G. arboreum, and expression analysis of its orthologs in G. hirsutum

Jianjiang Ma¹, Nuohan Wang^{1, 2}, Man Wu¹, Wenfeng Pei¹, Wenkui Wang¹, Xingli Li¹, Jinfa Zhang¹, Shuxun Yu^{1, 2}, Jiwen Yu¹

1. State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China;
2. College of Agronomy, Northwest A&F University, Yangling 712100, China

Received:2015-01-05 Revised:2015-05-14 Online:2015-07-20 Published:2015-07-20

Abstract

Abstract: Lysophosphatidic acid acyltransferase (LPAAT) which converts lysophosphatidic acid into phosphatidic acid is a key enzyme in biosynthesis pathway of lipid in plants. In this study, we identified 17 members of the LPAAT gene family from genomic data of G. raimondii-D₅ and G. arboreum-A₂. Analysis of gene structure, chromosome distribution and phylogenetic evolution of LPAAT genes in diploid Gossypium using bioinformatics approaches showed that these genes can be divided into distinct subfamilies based on the distance of their genetic relationship. Moreover, the gene structures were similar within LPAAT subfamily members. The amino acid sequences encoded by LPAAT family genes contained three conserved motifs, including ΦFPEGTR-G binding site and Φ-NHQS- ΦDΦΦ catalytic site. Phylogenetic analysis of LPAAT gene family demonstrated significant differences in evolution of LPAAT in different species. Finally, expression analysis of G. hirsutum ovules in different stages from RNA-seq and qRT-PCR data indicated that LPAAT gene may play a positive role in oil accumulation. Our studies facilitate understanding of the function of LPAAT gene family in Gossypium and selecting better LPAAT genes for further functional validation.

Key words: cottonseed oil, LPAAT family, gene structure, phylogenic analysis, expression analysis

Jianjiang Ma, Nuohan Wang, Man Wu, Wenfeng Pei, Wenkui Wang, Xingli Li, Jinfa Zhang, Shuxun Yu, Jiwen Yu. Genome-wide analysis of the LPAAT gene family in Gossypium raimondii and G. arboreum, and expression analysis of its orthologs in G. hirsutum[J]. HEREDITAS(Beijing), 2015, 37(7): 692-701.

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Genome-wide analysis of the LPAAT gene family in Gossypium raimondii and G. arboreum, and expression analysis of its orthologs in G. hirsutum

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