黄瓜单性花决定基因M的表达分析

doi:10.3724/SP.J.1005.2010.00632

[an error occurred while processing this directive]

HEREDITAS ›› 2010, Vol. 32 ›› Issue (6): 632-638.doi: 10.3724/SP.J.1005.2010.00632

• en • Previous Articles Next Articles

Expression analysis of the unisex-determine gene M in cucumber

TAO Qian-Yi, LI Zheng, HE Huan-Le, PAN Jun-Song, CAI Run

School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China

Received:2009-10-11 Revised:2010-03-04 Online:2010-06-20 Published:2010-05-24
Contact: CAI Run E-mail:cairun@sjtu.edu.cn

Abstract

Abstract:

Sex determination in plants has various machnisam; however, a single gene locus controlling unisexual expression is unique in the Cucurbitaceae plants, particularly cucumber (Cucumis sativus L.) and melon (Cucumis melo L.). In this study, with quantitative RT-PCR method, two sets of near-isogenic lines (NILs) were used to analyze the expression pattern of gene CsACS2 (GenBank accession number FJ529216). Additionally, chemical applications (AgNO₃ and AVG) were used to investi-gate the effect of the plant endogenous ethylene on CsACS2 expression. Expression analysis reveals that endogenous ethylene, which might be derived from F or M itself, could activate the expression of M gene.

Key words: Cucumis sativus L., sex determination, M gene, CsACS2, expression analysis

DAO Qian-Yi, LI Zheng, HE Huan-Le, BO Dun-Song, CA Run. Expression analysis of the unisex-determine gene M in cucumber[J]. HEREDITAS, 2010, 32(6): 632-638.

References

[1] Atsmon D. The interaction of genetic, environmental, and hormonal factors in stem elongation and floral develop-ment of cucumber plants. Ann Bot, 1968, 32(4): 877–882.

[2] Perl-Treves R. In: Ainsworth CC, ed. Sex Determination in Plants. BIOS, Oxford, 1999, 189–286.

[3] Tanurdzic M, Banks JA. Sex-determining mechanisms in land plants. Plant Cell, 2004, 16: s61–s71.

[4] Galun E. Study of the inheritance of sex expression in the cucumber: the interaction of major genes with modifying genetic and non-genetic factors. Genetica, 1961, 32(1): 134–163.

[5] Shifriss O. Sex control in cucumber. J Hered, 1961, 52(1): 5–12

[6] Kubicki B. Investigation of sex determination in cucumber (Cucumis sativus L.). Genet Pol, 1969, 10: 5–143. [7] Malepszy S, Niemirowicz-Szczytt K. Sex determination in cucumber (Cucumis sativus) as a model system for mo-lecular biology. Plant Sci, 1991, 80(4): 39–47.

[8] Robinson RW, Munger HM, Whitaker TW, Bohn GM. Genes of Cucubitaceae. Hort Sci, 1976, 11(2): 554–568. [9] Yin T, Quinn JA. Tests of a mechanistic model of one hormone regulating both sexes in Cucumis sativus (Cu-curbitaceae). Am J Bot, 1995, 82(12): 1537–1546.

[10] Yamasaki S, Fujii N, Matsuura S, Mizusawa H, Takahashi H. The M locus and ethylene-controlled sex determination in andromonoecious cucumber plants. Plant Cell Physiol, 2001, 42(6): 608–619.

[11] Trebitsh T, Staub JE, O’Neill SD. Identification of a 1-aminocyclopropane-1-carboxylic acid synthase gene linked to the female (F) locus that enhance female sex ex-pression in cucumber. Plant Physiol, 1997, 113(3): 987–995.

[12] Mibus H, Tatlioglu T. Molecular characterization and iso-lation of the F/f gene of femaleness in cucumber (Cucumis sativus L.). Theor Appl Genet, 2004, 109(8): 1669–1676.

[13] Knopf RR, Trebitsh T. The female-specific CS-ACS1G gene of cucumber. A case of gene duplication and recom-bination between the non-sex-specific 1-aminocyclopro- pane-1-carboxylate synthase gene and a branched-chain amino acid transaminase gene. Plant Cell Physiol, 2006, 47(9): 1217–1228.

[14] Li Z, Huang SW, Liu SQ, Pan JS, Zhang ZH, Tao QY, Shi QX, Jia ZQ, Zhang WW, Chen HM, Si LT, Zhu LH, Cai R. Molecular isolation of the M gene suggests that a con-served-residue conversion induces the formation of bi-sexual flowers in cucumber plants. Genetics, 2009, 182(4): 1381–1385.

[15] Boualem A, Troadec C, Kovalski I, Sari M-A, Perl-Treves R, Bendahmane A. A conserved ethylene biosynthesis en-zyme leads to andromonoecy in two cucumis species. PLoS ONE, 2009, 4(7): e6144.

[16] Saito S, Fujii N, Miyazawa Y, Yamasaki S, Matsuura S, Mizusawa H, Fujita Y, Takahashi H. Correlation between development of female flower buds and expression of the CS-ACS2 gene in cucumber plants. J Exp Bot, 2007, 58(11): 2897–2907.

[17] Li Z, Pan JS, Guan Y, Tao QY, He HL, Si LT, Cai R. De-velopment and fine mapping of three co-dominant SCAR markers linked to the M/m gene in the cucumber plant (Cucumis sativus L.). Theor Appl Genet, 2008, 117(8): 1253–1260.

[18] Yamasaki S, Fujii N, Takahashi H. The ethylene-regulated expression of CS-ETR2 and CS-ERS genes in cucumber plants and their possible involvement with sex expression in flowers. Plant Cell Physiol, 2000, 41(5): 608–616.

[19] Duan QH, Wang DH, Xu ZH, Bai SN. Stamen develop-ment in Arabidopsis is arrested by organ-specific overex-pression of a cucumber ethylene synthesis gene CsACO2. Planta, 2008, 228(4): 537–543.

Metrics

Comments

www.chinagene.cn
备案号：京ICP备09063187号

Expression analysis of the unisex-determine gene M in cucumber

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	Feifei Li, Yanmin Hao, Minlong Cui, Chunlan Piao. Cloning and functional analysis of RADIALIS-like 1 gene from Antirrhinum majus [J]. Hereditas(Beijing), 2023, 45(6): 526-535.
[2]	Qingqian Ding,Xiaoting Wang,Liqin Hu,Xin Qi,Linhao Ge,Weiya XU,Zhaoshi Xu,Yongbin Zhou,Guanqing Jia,Xianmin Diao,Donghong Min,Youzhi Ma,Ming Chen. MYB-like transcription factor SiMYB42 from foxtail millet (Setaria italica L.) enhances Arabidopsis tolerance to low-nitrogen stress [J]. Hereditas(Beijing), 2018, 40(4): 327-338.
[3]	Lizhen Zhang, Yong Zhang, Jinghua Hu, Zilong Wang, Zhijiang Zeng. Molecular cloning and expression analysis of the tyramine receptor genes in Apis cerana cerana [J]. Hereditas(Beijing), 2018, 40(2): 155-161.
[4]	Yi Kang,Guijun Guan,Yunhan Hong. Insights of sex determination and differentiation from medaka as a teleost model [J]. Hereditas(Beijing), 2017, 39(6): 441-454.
[5]	Jianzhong Chang, Fengxia Yan, Linyi Qiao, Jun Zheng, Fuyao Zhang, Qingshan Liu. Genome-wide identification and expression analysis of SBP-box gene family in Sorghum bicolor L. [J]. HEREDITAS(Beijing), 2016, 38(6): 569-580.
[6]	Yanbing Gu, Zhirui Ji, Fumei Chi, Zhuang Qiao, Chengnan Xu, Junxiang Zhang, Zongshan Zhou, Qinglong Dong. Genome-wide identification and expression analysis of the WRKY gene family in peach [J]. HEREDITAS(Beijing), 2016, 38(3): 254-270.
[7]	Jun Wu, Junhong Zhang, Menghui Huang, Minhui Zhu, Zaikang Tong. Expression analysis of miR164 and its target gene NAC1 in response to low nitrate availability in Betula luminifera [J]. HEREDITAS(Beijing), 2016, 38(2): 155-162.
[8]	Jianjiang Ma, Nuohan Wang, Man Wu, Wenfeng Pei, Wenkui Wang, Xingli Li, Jinfa Zhang, Shuxun Yu, Jiwen Yu. Genome-wide analysis of the LPAAT gene family in Gossypium raimondii and G. arboreum, and expression analysis of its orthologs in G. hirsutum [J]. HEREDITAS(Beijing), 2015, 37(7): 692-701.
[9]	Meng Li, Zhumei He. Elucidation of key genes in sex determination in genetics teaching [J]. HEREDITAS(Beijing), 2014, 36(6): 611-617.
[10]	Yunfei Liu, Hongjian Wan, Yuejian Yang, Yanping Wei, Zhimiao Li, Qingjing Ye, Rongqing Wang, Meiying Ruan, Zhuping Yao, Guozhi Zhou. Genome-wide identification and analysis of heat shock protein 90 in tomato [J]. HEREDITAS(Beijing), 2014, 36(10): 1043-1052.
[11]	HUANG Yi-Min XIA Meng-Ying HUANG Shi. Evolutionary process unveiled by the maximum genetic diversity hypothesis [J]. HEREDITAS, 2013, 35(5): 599-606.
[12]	HUANG Guo-Wen HAN Yu-Zhen FU Yong-Fu. Expression and functional analysis of SUA41gene in Arabidopsis thaliana [J]. HEREDITAS, 2013, 35(1): 93-100.
[13]	YANG Li-Wei, SHI Ji-Sen. Cloning and expression analysis of differentially expressed genes in Chinese fir stems treated by different concentrations of exogenous IAA [J]. HEREDITAS, 2012, 34(4): 472-484.
[14]	YANG Xiu-Rong, JIANG He-Sheng, YANG Ning. Mechanism of avian sex determination and differentiation [J]. HEREDITAS, 2012, 34(4): 407-411.
[15]	JIAO Sha-Sha, LIU Ka, LI Gang, GAO Jian-Feng, MA Run-Lin. Confirmation and expression analysis of three predicted genes in sheep MHC region [J]. HEREDITAS, 2011, 33(12): 1353-1358.