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HEREDITAS ›› 2010, Vol. 32 ›› Issue (5): 498-504.doi: 10.3724/SP.J.1005.2010.00498

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Cloning and functional analysis of trehalose -6-phosphate synthase gene from Selaginella pulvinata

LIN Jing, FU Feng-Ling, JIANG Wei, MU Yu, YONG Tai-Ming, LI Wan-Chen   

  1. Maize Research Institute, Sichuan Agricultural University, Ya’an 625014, China
  • Received:2009-10-09 Revised:2009-12-02 Online:2010-05-20 Published:2010-05-15
  • Contact: LI Wan-Chen E-mail:aumdyms@sicau.edu.cn

Abstract:

Trehalose-6-phosphate synthase, a key enzyme in trehalose synthesis pathway of plant, plays an important role in response to abiotec stress in xerophilous rock lily and other resurrection plants. In this study, homologous amplification and RACE technique were used to clone gene SpTPS1 for trehalose-6-phosphate synthase from Selaginella pulvinata, which is an endemic xerophilous plant in China. The full-length cDNA is 3 223 bp long, containing an open reading frame (ORF) of 2 790 bp. Protein sequence comparison showed that the pupative amino acid sequence of this ORF shares high similarity to trehalose-6-phosphate synthases of mode speciese, especially at the conserved sites of catalytic activity centers. Yeast functinal complementation test showed that trehalose-6-phosphate synthase mutant (tps1△), transformed by the ORF of SpTPS1 gene, can restore growth on the mediam supplemented with glucose as a sole carbon source. This result indicated that SpTPS1 of S. pulvinata encodes for an activative protein and is hopeful to be applied in transgeneic improvement of abiotic stress tolerance in plant.

Key words: Selaginella pulvinata, trehalose-6-phosphate synthase, gene cloning, function