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HEREDITAS ›› 2011, Vol. 33 ›› Issue (12): 1327-1334.doi: 10.3724/SP.J.1005.2011.01327

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T-DNA integration patterns in transgenic plants mediated by Agrobacterium tumefaciens

YANG Lin, FU Feng-Ling, LI Wan-Chen   

  1. Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, China
  • Received:2011-03-30 Revised:2011-07-25 Online:2011-12-20 Published:2011-12-25

Abstract: The genetic transformation mediated by Agrobacterium tumefaciens has been widely applied to research of transgenic plants. As the vector of the exotic genes, the integration patterns of T-DNA fragments affects not only transformation efficiency and stability, but also expression properties of the transgenes. This review summaries the two major patterns and the rules of T-DNA integration in Agrobacterim-mediated transformation, rules of T-DNA me-diated by Agrobacterium tumefaciens, as well as research tools for flanking sequence amplification. It is attempted to provide references for researches on transformation and T-DNA integration mutation mediated by Agrobacterium tumefaciens.

Key words: Agrobacterium tumefaciens, transfer DNA, flanking sequence, integration, transgene