[an error occurred while processing this directive]

HEREDITAS ›› 2011, Vol. 33 ›› Issue (12): 1317-1326.doi: 10.3724/SP.J.1005.2011.01317

• en • Previous Articles     Next Articles

Application of next generation sequencing techniques in plant transcriptome

LIANG Ye, CHEN Shuang-Yan, LIU Gong-She   

  1. The Research and Development Laboratory for Plant Resources, Institute of Bontany, Chinese Acdemy of Sciences, Beijing 100093, China
  • Received:2010-11-16 Revised:2011-08-24 Online:2011-12-20 Published:2011-12-25

Abstract: With the development of DNA sequencing techniques, the next-generation sequencing (NGS) techniques with the characteristics of high-throughput and low cost have become the first choice for more and more researchers to carry out the biological researches. Among the next-generation sequencing techniques, the 454 sequencing platform is the first com-mercially available and relatively mature one and widely used in various fields of biological research. Taking 454 sequenc-ing platform as an example, we illustrate the advantages and disadvantages of NGS technical principles, review their appli-cations in plant transcriptome, and outlook their future development and applications in plant research field.

Key words: EST, SNP, next generation sequencing techniques (NGS), 454 pyrosequencing technology, plant transcriptome