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HEREDITAS ›› 2012, Vol. 34 ›› Issue (7): 857-862.doi: 10.3724/SP.J.1005.2012.00857

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Progression on genetic knockout tools in Mycobacterium

DU Qin-Lin, FAN Xiang-Yu, MAO Jin-Xiao, XIE-Jian-Ping   

  1. Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, School of Life Sciences, Southwest University, Chongqing 400715, China
  • Received:2011-12-07 Revised:2012-04-04 Online:2012-07-20 Published:2012-07-25

Abstract: Pathogenic mycobacteria were and remain a heavy burden to public health. Unfortunately, genetic manipulation including knockout technologies of Mycobacterium is difficult compared with other traditional model organisms. To overcome this obstacle, achievements in Mycobacterium knockout technologies were summarized, including deliv-ery vector, sequence-specific recombination system, as well as the recently developed recombinogenic engineering and its application. The future for this tool innovation is also addressed.

Key words: Mycobacterium, knockout, delivery vector, sequence-specific recombinase, recombinogenic engineering