建鲤肠型脂肪酸结合蛋白基因的分离及其SNPs与增重的相关分析

doi:10.3724/SP.J.1005.2013.00628

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HEREDITAS ›› 2013, Vol. 35 ›› Issue (5): 628-636.doi: 10.3724/SP.J.1005.2013.00628

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Sequences analysis of jlFABP2 and the correlation between polymorphisms and body weight gain in Cyprinus carpio var. jian

XIA Zheng-Long¹, YU Ju-Hua^1,2, LI Hong-Xia², LI Jian-Lin², TANG Yong-Kai², REN Hong-Tao³, ZHU Shuang-Ning¹

1. Wuxi Fishery College, Nanjing Agricultural University, Wuxi 214081, China 2. Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China 3. Animal Science and Technology College, Henan University of Science and Technology, Luoyang 471003, China

Received:2012-09-23 Revised:2013-11-12 Online:2013-05-20 Published:2013-05-25

Abstract

Abstract: Two replicate intestine fatty acid binding protein genes (jlFABP2a and jlFABP2b) were cloned from Cyprinus carpio var. jian using PCR. Both ORFs were 399 bp in length sharing 92.2% similarity with each other, and 88.0% and 90.5% with their counterpart in zebrafish, respectively. The gene structure of jlFABP2s was same as other FABPs, which contained four exons and three introns. Sequences and lengths of introns between 2a and 2b. were obviously different Phylogenetic tree displayed that two jlFABP2s corresponded to one zebrafish FABP2 which matches the fact that the chromosome number of common carp was twice of zebrafish. Real time-PCR showed that jlFABP2 genes mainly expressed in intestine and the expression level was very significantly higher than other tissues such as brain, liver, muscle, kidney, and gonad (P<0.01). The expression level of jlFABP2a was significantly (male, P<0.05) or very significantly (females, P<0.01) higher than 2b in intestine; and 2b was expressed slightly higher than 2a in other tissues. It seemed that 2a expressed specifically in intestine, while 2b expressed ubiquitously. Twelve and four SNP loci were found at jlFABP2a and 2b introns through comparison sequences from 8 individuals, respectively. Geno-types of I1-A15G, I1-A99G, I2-C487T, and I3-A27T on jlFABP2a were detected using PCR-RFLP in selection population of C. carpio var. jian. The SNP genotypes and individual weight gain correlation indicated that four SNPs were significantly (P<0.05 or P<0.01) associated with adult weight gain. Diplotype analysis displayed that individuals with genotype AGGGCCXX or AGGGXXAT grew faster than other individuals by 15%. The individuals with these two genotypes only occupied 9% in total selection populations, indicating the presence of large selec-tion space. The 4 SNPs detected in this experiment can be used in C. carpio var. Jian growth selection breeding plan.

Key words: Cyprinus carpio var. jian, FABP2, tissue expression, single nucleotide polymorphisms loci, SNP-weight gain association analysis

XIA Zheng-Long YU Ju-Hua LI Hong-Xia LI Jian-Lin TANG Yong-Kai REN Hong-Tao ZHU Shuang-Ning. Sequences analysis of jlFABP2 and the correlation between polymorphisms and body weight gain in Cyprinus carpio var. jian[J]. HEREDITAS, 2013, 35(5): 628-636.

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Sequences analysis of jlFABP2 and the correlation between polymorphisms and body weight gain in Cyprinus carpio var. jian

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