山羊CAST基因Ⅱ型转录本的克隆及在山羊不同组织中的表达

doi:10.3724/SP.J.1005.2011.00358

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HEREDITAS ›› 2011, Vol. 33 ›› Issue (4): 358-364.doi: 10.3724/SP.J.1005.2011.00358

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Cloning and tissue expression of CAST transcriptⅡ in goat

ZHAO Bo-Yang¹, WANG Dai-Hua¹, XU Gang-Yi¹, ZHAO Wen-Bei², ZHENG Cheng-Li¹

1. Research Institute of Animal Genetics and Breeding, Sichuan Agricultural University, Ya’an 625014, China 2. Yanting Huiyuan livestock Co., Ltd, Yanting, Sichuan Province 621600, China

Received:2010-08-10 Revised:2010-10-02 Online:2011-04-20 Published:2011-04-25
Contact: XU Gang-Yi E-mail:goatsxgy@263.net

Abstract

Abstract: Calpastatin (CAST) gene is closely related with meat quality in livestock and poultry. Based on the bovine and ovine mRNA sequences, the cDNA of CAST Ⅱgene in goat was amplified successfully for the first time by using RACE-PCR. Results showed that CASTⅡof goat was 2474 bp in length with an open reading frame (ORF) 1695 bp long and encoded 564 amino acids, and there were four conserved domains and one conserved seven-peptide do-main in amino acids sequences. Bioinformation analysis indicated that its secondary structures mainly were random coil and helical regions, and contained rich hydrophobic regions, certain phosphorylation sites, and protein kinase C (PKC) sites. Meanwhile, analysis of tissue expression of the gene in Tianfu meat goat demonstrated it was expressed in seven selected tissues. When the goat was of 6-month age, the highest expression was observed in longissimusdorsi, which was significantly higher than that of crureus (P<0.05) and other internal organ tissues (P<0.01). Furthermore, the expression of CAST II increased with the rise of the age and became the highest when the goat was at three-year age.

Key words: CASTⅡ gene, RACE, tissue expression, goat, cDNA clone

ZHAO Bo-Yang, WANG Dai-Hua, XU Gang-Yi, ZHAO Wen-Bei, ZHENG Cheng-Li. Cloning and tissue expression of CAST transcriptⅡ in goat[J]. HEREDITAS, 2011, 33(4): 358-364.

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Cloning and tissue expression of CAST transcriptⅡ in goat

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