结合SSR标记和STS标记对家蚕无鳞毛翅基因的定位

doi:10.3724/SP.J.1005.2010.00054

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HEREDITAS ›› 2010, Vol. 32 ›› Issue (1): 54-58.doi: 10.3724/SP.J.1005.2010.00054

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Mapping of non-lepis wing gene nlw in silkworm (Bombyx mori) using SSR and STS markers

WANG Xiu-Ye¹, LI Mu-Wang^{1, 2}, ZHAO Yun-Po³, XU An-Ying^{1, 2}, GUO Qiu-Hong³, HUANG Yong-Ping³,
GUO Xi-Jie^{1, 2}

1. Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China; 2. College of Biotechnology and Environmental Engineering, Jiangsu University of Science and Technology, Zhenjiang 212018, China; 3. Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China

Received:2009-05-26 Revised:2009-09-13 Online:2010-01-20 Published:2010-01-15
Contact: LI Mu-Wang E-mail:muwang_li@hotmail.com

Abstract

Abstract:

The non-lepis wing of silkworm (Bombyx mori) is controlled by the recessive gene, nlw. Owning to lack of crossing over in females, the reciprocal backcrossed F₁ (BC₁) progenies were used for linkage analysis and mapping of nlw based on the SSR linkage map and STS markers using the wild type (+^nlw/+^nlw) silkworm strain P50 and U06 with scaleless wing (nlw/nlw). The nlw gene was linked to eight SSR markers and one STS marker. All the individuals with the wild type in the BC₁F (Using F₁ as female to backcross to the recessive parent, that is (U06×P50)×U06) showed heterozygous profile of (U06×P50) F₁, and the ones with non-lepis wing in BC₁F exhibited the homozygous profile of the strain U06. Using a reciprocal BC₁M (Using F₁ as male to backcross to the recessive parent, that is U06×(U06×P50))cross, we constructed a linkage map of 125.6 cM, and the distance between nlw and the nearest marker cash2p was 11.4 cM.

Key words: Bombyx mori, non-lepis wing, Gene linkage map, SSR, STS

WANG Xiu-Ye, LI Mu-Wang, DIAO Yun-Po, XU An-Yang, GUO Qiu-Gong, HUANG Yong-Beng, GUO Ti-Jie. Mapping of non-lepis wing gene nlw in silkworm (Bombyx mori) using SSR and STS markers[J]. HEREDITAS, 2010, 32(1): 54-58.

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Mapping of non-lepis wing gene nlw in silkworm (Bombyx mori) using SSR and STS markers

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