人精母细胞重组频率和年龄相关性的分析

doi:10.3724/SP.J.1005.2011.00725

[an error occurred while processing this directive]

HEREDITAS ›› 2011, Vol. 33 ›› Issue (7): 725-730.doi: 10.3724/SP.J.1005.2011.00725

• en • Previous Articles Next Articles

Correlation analysis between meiotic recombination frequencies and age in human spermatocyte

DUAN Tao¹, YANG Qing-Ling², WANG Liu², SHI Qing-Hua², YU De-Xin¹

1. Department of Urology, First Affiliated Hospital, Anhui Medical University, Hefei 230022, China 2. Hefei National Laboratory for Physical Sciences at Microscale, School of Life Science, University of Science Technology of China, Hefei 230027, China

Received:2011-03-16 Revised:2011-04-09 Online:2011-07-20 Published:2011-07-25

Abstract

Abstract: Faithful meiotic recombination is essential for the segregation of homologous chromosomes and the formation of normal haploid gametes. Little is known about the mechanism of meiotic recombination in human germ cells. MLH1 (a DNA mismatch repair protein) foci on synaptonemal complexes (SCs) at prophase I of meiosis can be used to examine re-combination frequency. In 10 fertile men, the mean number of MLH1 foci per cell in all donors was 49.4 with a range from 33 to 63. There was significant variation in the recombination frequency found among 10 normal individuals: the mean frequencies of chromosomal recombination foci ranged from 47 to 52.7. The bivalents without recombination focus were rare, with a frequency of only 0.4%. Thus, achiasmate chromosomes appeared to be rare in human male meiosis. Spearman correlation analysis between age and the frequencies of recombination foci failed to get any significantly statistical correla-tion, suggesting that aging contributes nothing to the variation among individuals.

Key words: meiosis, synaptonemal complex, genetic recombination, spermatocyte, age

DUAN Chao, YANG Qiang-Ling, WANG Liu, SHI Qiang-Hua, XU De-Xin. Correlation analysis between meiotic recombination frequencies and age in human spermatocyte[J]. HEREDITAS, 2011, 33(7): 725-730.

References

[1] Hassold T, Sherman S, Hunt P. Counting cross-overs: characterizing meiotic recombination in mammals. Hum Mol Genet, 2000, 9(16): 2409-2419.
[2] von Wettstein D, Rasmussen SW, Holm PB. The synaptonemal complex in genetic segregation. Annu Rev Genet, 1984, 18(1): 331-413.
[3] 刘静宇, 张传善. 褐家鼠精母细胞中联会复合体的研究Ⅰ. 表面铺展联会复合体的PTA染色. 遗传, 1995, 17(2): 17-19.
[4] Li J, Leng M, Ma T, Yu D, Shi H, Shi Q. Cryopreservation has no effect on meiotic recombination and synapsis in testicular tissues. Fertil Steril, 2009, 91(Suppl.4): 1404-1407.
[5] Tease C, Hartshorne GM, Hultén MA. Patterns of meiotic recombination in human fetal oocytes. Am J Hum Genet, 2002, 70(6): 1469-1479.
[6] Baker SM, Plug AW, Prolla TA, Bronner CE, Harris AC, Yao X, Christie DM, Monell C, Arnheim N, Bradley A, Ashley T, Liskay RM. Involvement of mouse Mlh1 in DNA mismatch repair and meiotic crossing over. Nat Genet, 1996, 13(3): 336-342.
[7] Barlow AL, Hultén MA. Crossing over analysis at pachytene in man. Eur J Hum Genet, 1998, 6(4): 350-358.
[8] Sun F, Trpkov K, Rademaker A, Ko E, Martin RH. Variation in meiotic recombination frequencies among human males. Hum Genet, 2005, 116(3): 172-178.
[9] Lyrakou S, Mantas D, Msaouel P, Baathalah S, Shrivastav P, Chrisostomou M, Mihalopoulos Y, Hasiakos D, Baka S. Crossover analysis using immunofluorescent detection of MLH1 foci in frozen-thawed testicular tissue. Reprod Biomed Online, 2007, 15(1): 99-105.
[10] Sun F, Oliver-Bonet M, Liehr T, Starke H, Turek P, Ko E, Rademaker A, Martin RH. Variation in MLH1 distribution in recombination maps for individual chromosomes from human males. Hum Mol Genet, 2006, 15(15): 2376-2391.
[11] Lynn A, Koehler KE, Judis L, Chan ER, Cherry JP, Schwartz S, Seftel A, Hunt PA, Hassold TJ. Covariation of synaptonemal complex length and mammalian meiotic exchange rates. Science, 2002, 296(5576): 2222-2225.
[12] Codina-Pascual M, Campillo M, Kraus J, Speicher MR, Egozcue J, Navarro J, Benet J. Crossover frequency and synaptonemal complex length: their variability and effects on human male meiosis. Mol Hum Reprod, 2006, 12(2): 123-133.
[13] Jagiello G, Fang JS. Analyses of diplotene chiasma frequencies in mouse oocytes and spermatocytes in relation to ageing and sexual dimorphism. Cytogenet Cell Genet, 1979, 23(1-2): 53-60.
[14] Luthardt FW, Palmer CG, Yu P. Chiasma and univalent-frequencies in aging female mice. Cytogenet Cell Genet, 1973, 12(1): 68-79.
[15] Speed RM. The effects of ageing on the meiotic chromosomes of male and female mice. Chromosoma, 1977, 64(3): 241-254.
[16] Tanzi RE, Watkins PC, Stewart GD, Wexler NS, Gusella JF, Haines JL. A genetic linkage map of human chromosome 21: analysis of recombination as a function of sex and age. Am J Hum Genet, 1992, 50(3): 551-558.
[17] Shi QH, Spriggs E, Field LL, Rademaker A, Ko E, Barclay L, Martin RH. Absence of age effect on meiotic recombination between human X and Y chromosomes. Am J Hum Genet, 2002, 71(2): 254-261.
[18] Ferguson KA, Wong EC, Chow V, Nigro M, Ma S. Abnormal meiotic recombination in infertile men and its association with sperm aneuploidy. Hum Mol Genet, 2007, 16(23): 2870-2879.
[19] Bascom-Slack CA, Ross LO, Dawson DS. Chiasmata, crossovers, and meiotic chromosome segregation. Adv Genet, 1997, 35: 253-284.
[20] Roeder GS, Bailis JM. The pachytene checkpoint. Trends Genet, 2000, 16(9): 395-403.
[21] Shi QH, Spriggs E, Field LL, Ko E, Barclay L, Martin RH. Single sperm typing demonstrates that reduced recombi-nation is associated with the production of aneuploid 24, XY human sperm. Am J Med Genet, 2001, 99(1): 34-38.
[22] Gonsalves J, Sun F, Schlegal PN, Hopps CV, Turek PJ, Greene C, Martin RH, Reijo-Pera RA. Defective recom-bination in infertile men. Hu

Metrics

Comments

www.chinagene.cn
备案号：京ICP备09063187号

Correlation analysis between meiotic recombination frequencies and age in human spermatocyte

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	Qingyu Sun, Yang Zhou, Lijuan Du, Mengke Zhang, Jiale Wang, Yuanyuan Ren, Fang Liu. Analysis between macrophage-related genes with prognosis and tumor microenvironment in non-small cell lung cancer [J]. Hereditas(Beijing), 2023, 45(8): 684-699.
[2]	Zimei Yang, Ge Zhang, Gang Wei, Lili Jing, Ming Yu. AFF4 globally affects the release of paused RNA polymerase II in HEL cells [J]. Hereditas(Beijing), 2023, 45(8): 658-668.
[3]	Xiangjiang Lv, Jing Guo, Ge Lin. Novel mutations in TRIP13 lead to female infertility with oocyte maturation arrest [J]. Hereditas(Beijing), 2023, 45(6): 514-525.
[4]	Nan Zhang, Jue Zhang, Ge Lin. Advances in the study of DNA damage and repair in mammalian oocytes [J]. Hereditas(Beijing), 2023, 45(5): 379-394.
[5]	Biyuan Li, Yanting Zhao, Zhichen Yue, Juanli Lei, Qizan Hu, Peng Tao. Identification of DELLA gene family in head cabbage and analysis of mRNA transport in the heterograft [J]. Hereditas(Beijing), 2023, 45(2): 156-164.
[6]	Tian Xie, Mei Wang, Ruiyu Gao, Yanni Miao, Yiming Zhang, Jing Jiang. Development and application of light-controlled inducible recombination systems [J]. Hereditas(Beijing), 2022, 44(8): 655-671.
[7]	Chong Zhang, Zixuan Wei, Min Wang, Yaosheng Chen, Zuyong He. Editing MC1R in human melanoma cells by CRISPR/Cas9 and functional analysis [J]. Hereditas(Beijing), 2022, 44(7): 581-590.
[8]	Jianmei Wang, Hehe Liu, Shengchao Ma, Yang Xi, Rongping Zhang, Qian Xu, Liang Li. Progress on the formation mechanism of sexual dimorphism plumage color in birds [J]. Hereditas(Beijing), 2022, 44(6): 491-500.
[9]	Yuxuan Guo, Shunping Yan, Yingxiang Wang. Recent advances in functional conservation and divergence of recombinase RAD51 and DMC1 [J]. Hereditas(Beijing), 2022, 44(5): 398-413.
[10]	Bingru Yan, Xianhui Ai, Miao Liu, Lihong Tian, Yang Liang, Chun-Bo Teng. Study on Lgr5 expression in pancreas tissues and organoids by lineage tracing [J]. Hereditas(Beijing), 2022, 44(5): 432-441.
[11]	Zhilu Fan, Liyan Yang, Na Zhang, Dan Feng, Jing Guo, Chen Chang, Qing Yuan, Yan Cai, Yu Zhang, Wenqiang Wei, Mingrong Wang, Jiajie Hao. NEFL promotes invasion and migration of esophageal squamous carcinoma cells via the EGFR/AKT/S6 pathway [J]. Hereditas(Beijing), 2022, 44(4): 322-334.
[12]	Yizhun Zeng, Tao Zhang, Ying Xu. Rapid assessment of circadian behavior in mice [J]. Hereditas(Beijing), 2022, 44(4): 346-357.
[13]	Wanzi Jiang, Yiwen Xu, Yiwen Wang, Xiaocheng Zhu, Yingyun Gong, Hongwen Zhou, Zhenzhen Fu. Diagnosis and genetic analysis of a case with glycogen storage disease type V caused by compound heterozygous mutations in the PYGM gene [J]. Hereditas(Beijing), 2022, 44(11): 1063-1071.
[14]	Tingting Jia, Lei Lei, Xinyuan Wu, Shunyou Cai, Yixuan Chen, Yu Xue. Study on the mechanism of metformin on zebrafish skeletal development and damage repair [J]. Hereditas(Beijing), 2022, 44(1): 68-79.
[15]	Yuanyuan Li, Lei Guo, Zhiming Han. Roles of NEK family in cell cycle regulation [J]. Hereditas(Beijing), 2021, 43(7): 642-653.