奶山羊PIS遗传缺陷基因的检测及应用

doi:10.3724/SP.J.1005.2012.00895

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HEREDITAS ›› 2012, Vol. 34 ›› Issue (7): 895-900.doi: 10.3724/SP.J.1005.2012.00895

Detection and application of PIS genetic deficiency gene in dairy goat

YANG Bo^1,2, JIA Li-Li^1,2, ZHAO De-Chao^1,2, MENG Li-Yun^1,2, LIU Xue-Feng³, ZHANG Yan-Jun^1,2, ZHANG Wen-Guang^1,2,4, LI Jin-Quan^1,2

1. College of AnimalScience, Inner Mongolia Agricultural University, Hohhot 010018, China 2. Key Laboratory of Animal Genetics, Breeding and Reproduction of the NeiMongol Autonomous Region, Hohhot 010018, China 3. College of Life Science, Inner Mongolia Agricultural University, Hohhot 010018, China 4. Institute of ATCG, Nei Mongol Bio-Information, Hohhot 010020, China

Received:2011-12-29 Revised:2012-04-10 Online:2012-07-20 Published:2012-07-25

Abstract

Abstract: The purpose of this study was to develop a molecular method for detecting polled intersex syndrome (PIS) genetic deficiency gene in dairy goat. Three pairs of primers, PIS^-, PIS⁺, and NEI were designed based on PIS gene sequence (AF404302) to identify the PIS genetic deficiency genotype. For the normal phenotype, the fragments of 141 and 300 bp were obtained for the genotype PIS^-PIS^-, and 141, 449, and 300 bp for the genotype PIS-PIS+. For the PIS goat with the genotype PIS^⁺PIS⁺, 449 and 300 bp were obtained. Two hundred and twenty-four dairy goats in one population were tested based on this method. The results showed that there were 150 PIS^-PIS⁺, 70 PIS^-PIS^-, and 4 PIS⁺PIS⁺. The genotype frequency of PIS^-PIS⁺ was 66.9%, and the gene frequency of PIS⁺ was 35.3% in the population. Therefore, the frequency of PIS offspring was over 12%. This study developed a method to detect PIS genetic deficiency dairy goat. The method could identify buck genotype accurately to avoid the occur-rence of PIS genetic deficiency. The ease and accuracy show a strong potential of the method for use in marker assisted selection of dairy goats and healthy development of dairy goat industry.

Key words: polled intersex syndrome, molecular genetic testing, genotyping, sex identifying

YANG Bo, JIA Li-Li, ZHAO De-Chao, MENG Li-Yun, LIU Xue-Feng, ZHANG Yan-Jun, ZHANG Wen-Guang, LI Jin-Quan. Detection and application of PIS genetic deficiency gene in dairy goat[J]. HEREDITAS, 2012, 34(7): 895-900.

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Detection and application of PIS genetic deficiency gene in dairy goat

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