哺乳动物基因组靶向修饰阳性细胞富集的报告载体系统研究进展

doi:10.16288/j.yczz.15-247

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HEREDITAS(Beijing) ›› 2016, Vol. 38 ›› Issue (1): 28-39.doi: 10.16288/j.yczz.15-247

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Research progress in developing reporter systems for the enrichment of positive cells with targeted genome modification

Yichun Bai¹, Kun Xu¹, Zehui Wei¹, Zheng Ma², Zhiying Zhang¹

1. College of Animal Science & Technology, Northwest A&
F University, Yangling 712100, China;
2. Xingping Animal Husbandry Bureau of Shaanxi Province,Xingping 713199, China

Received:2015-06-01 Revised:2015-10-30 Online:2016-01-20 Published:2016-01-20
Supported by:
[Supported by the National Basic Research Program of China (973 Program) (No; 2011CBA01002) and the National Science and Technology Major Project of China (No; 2014ZX0801009B)]

Abstract

Abstract: Targeted genome editing technology plays an important role in studies of gene function, gene therapy and transgenic breeding. Moreover, the efficiency of targeted genome editing is increased dramatically with the application of recently developed artificial nucleases such as ZFNs, TALENs and CRISPR/Cas9. However, obtaining positive cells with targeted genome modification is restricted to some extent by nucleases expression plasmid transfection efficiency, nucleases expression and activity, and repair efficiency after genome editing. Thus, the enrichment and screening of positive cells with targeted genome modification remains a problem that need to be solved. Surrogate reporter systems could be used to reflect the efficiency of nucleases indirectly and enrich genetically modified positive cells effectively, which may increase the efficiency of the enrichment and screening of positive cells with targeted genome modification. In this review, we mainly summarized principles and applications of reporter systems based on NHEJ and SSA repair mechanisms, which may provide references for related studies in future.

Key words: targeted genome modification, engineered nucleases, surrogate reporter system, enrichment of positive cells

Yichun Bai, Kun Xu, Zehui Wei, Zheng Ma, Zhiying Zhang. Research progress in developing reporter systems for the enrichment of positive cells with targeted genome modification[J]. HEREDITAS(Beijing), 2016, 38(1): 28-39.

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Research progress in developing reporter systems for the enrichment of positive cells with targeted genome modification

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