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HEREDITAS ›› 2013, Vol. 35 ›› Issue (4): 395-409.doi: 10.3724/SP.J.1005.2013.00395

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TALE nuclease engineering and targeted genome modification

SHEN Yan, XIAO An, HUANG Peng, WANG Wei-Ye, ZHU Zuo-Yan, ZHANG Bo   

  1. Key Laboratory of Cell Proliferation and Differentiation of Ministry of Education, College of Life Sciences, Peking University, Beijing 100871, China
  • Received:2012-11-12 Revised:2013-01-24 Online:2013-04-20 Published:2013-04-25

Abstract: Artificial designer nucleases targeting specific DNA sequences open up a new field for reverse genetics study. The rapid development of engineered endonucleases (EENs) enables targeted genome modification theoretically in any species. The construction of transcription activator-like effector nucleases (TALENs) is simpler with higher specificity and less toxicity than zinc-finger nucleases (ZFNs). Here, we summarized the recent progresses and prospects of TALEN technology, with an emphasis on its structure, function, and construction strategies, as well as a collection of species and genes that have been successfully modified by TALENs, especially the application in zebrafish.

Key words: transcription activator-like effector (TALE), transcription activator-like effector nuclease (TALEN), engineered endonuclease (EEN), genome editing, targeted genome modification