Ds插入突变体的遗传学综合性实验设计与探讨

doi:10.16288/j.yczz.19-149

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Hereditas(Beijing) ›› 2019, Vol. 41 ›› Issue (12): 1148-1155.doi: 10.16288/j.yczz.19-149

• Genetics Teaching • Previous Articles

Design and exploration of genetic comprehensive experiments based on Ds insertion mutants

Nan Li¹, Yajuan Li¹, Haibin Guo¹, Xiangqian Zhang²()

^1. Center of Experimental Teaching for Common Basic Courses, South China Agricultural University, Guangzhou 510642, China
^2. College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China

Received:2019-09-26 Revised:2019-11-01 Online:2019-12-20 Published:2019-11-06
Contact: Zhang Xiangqian E-mail:aacrav@163.com
Supported by:
Supported by the National Natural Science Foundation of China Nos(30900884);Supported by the National Natural Science Foundation of China Nos(31671594);Teaching Reform and Research Projects of SCAU in 2017 No(JG17093)

Abstract

Abstract:

The cultivation of innovative abilities has become an important guide for higher education in China. Strengthening the integrated knowledge to design experiments is an effective way to improve undergraduate students’ innovative abilities. Herein we designed a comprehensive experiment for molecular genetics by utilizing a rice Ds insertion mutant identified previously in our research project. In the comprehensive experiment, we adopt the method of scientific research as the main line of teaching and take the interesting phenotype of the rice mutant as the breakthrough point to reform and innovate genetics laboratory teaching. On the basis of this, we combined the progressive teaching method and guided the students to learn the TAIL-PCR skill and conduct an innovative experiment through expanding their knowledge. The comprehensive experiment will deepen students’ understandings of the relationship between genotypes and phenotypes, help them master the effective way of thinking and technologies for scientific research to further improve their ability of the integrated application capability of theory and practices.

Key words: comprehensive experiment, experimental teaching, gene mutation

Nan Li, Yajuan Li, Haibin Guo, Xiangqian Zhang. Design and exploration of genetic comprehensive experiments based on Ds insertion mutants[J]. Hereditas(Beijing), 2019, 41(12): 1148-1155.

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References 15

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引物	引物序列(5′→3′)	实验目的	备注
P10	TCCCGTCCGATTTCGACTTTA	Ds转座检测
Tra4	TAGCTCACTCATTAGGCACCC	Ds转座检测
P5	AAGCTCAAGCTGCTCTAGCATTCG	Ds转座检测
Ds3′-1a	GGTTCCCGTCCGATTTCGACT	TAIL-PCR	DsR-primer
Ds3′-2a	CGATTACCGTATTTATCCCGTTC	TAIL-PCR	DsR-primer
Ds3′-3a	TCGTTTCCGTCCCGCAAGT	TAIL-PCR	DsR-primer
AD3	WGTGNAGWANCANAGA	TAIL-PCR	AD primer
AD13	NTSGASNTCNGAATCA	TAIL-PCR	AD primer

Design and exploration of genetic comprehensive experiments based on Ds insertion mutants

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