表观遗传学综合性实验设计与探讨

doi:10.16288/j.yczz.21-294

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Hereditas(Beijing) ›› 2021, Vol. 43 ›› Issue (12): 1179-1187.doi: 10.16288/j.yczz.21-294

• Genetics Teaching • Previous Articles

Design and exploration of epigenetic comprehensive experiments

Xiangqian Zhang¹(), Nan Li², Xinming Xie¹

1. College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China
2. Experimental basis and practical training center, South China Agricultural University, South China Agricultural University, Guangzhou 510642, China

Received:2021-08-10 Revised:2021-10-10 Online:2021-12-20 Published:2021-10-21
Supported by:
Supported by the National Natural Science Foundation of China No(31671594);Teaching Reform and Research Projects of South China Agricultural University Nos(JG19104);Teaching Reform and Research Projects of South China Agricultural University Nos(JG20046)

Abstract

Abstract:

Epigenetics is the necessary content to constitute a complete genetic framework, and has become one of the focuses and difficulties of modern genetics education. Here we design a comprehensive experiment for epigenetics by utilizing a rice epigenetic short panicle (esp) mutant identified previously in our research project. The esp mutants show a shortened and compressed panicle phenotype, which is associated with hypomethylation in the transcriptional termination region of ESP gene, causing ESP overexpression in the esp mutants. This experiment uses mutant phenotypic observation as a starting point, integrates scientific research and course teaching, course experiment and open experiment, and demonstrates to students the full picture of a typical epigenetic science research and leading technology. The comprehensive experiment will deepen students’ understanding of the relationship between phenotypes and genotypes, epigenetic modification and gene expression, and help them master the effective way of thinking for scientific research to further improve their ability of innovative abilities.

Key words: epigenetics, comprehensive experiment, experimental teaching, DNA methylation

Xiangqian Zhang, Nan Li, Xinming Xie. Design and exploration of epigenetic comprehensive experiments[J]. Hereditas(Beijing), 2021, 43(12): 1179-1187.

Figures/Tables 4

Table 1

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References 14

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引物名称	引物序列(5′→3′)	位置/基因	实验目的	标记类型
RM140	F: TGCCTCTTCCCTGGCTCCCCTG	Chr.1	图位克隆	SSR
RM140	R: GGCATGCCGAATGAAATGCATG	Chr.1	图位克隆	SSR
RM466	F: TCCATCACCACATTCCCC	Chr.1	图位克隆	SSR
RM466	R: ACCCTTCTCTCGCTCTCTCC	Chr.1	图位克隆	SSR
PSM377	F: CTCACAAGAACGCCACAAAA	Chr.3	图位克隆	SSR
PSM377	R: TGACCAGAAAGGAGAAGGAAA	Chr.3	图位克隆	SSR
PSM382	F: GCAAGGGACCAACCAACCT	Chr.4	图位克隆	SSR
PSM382	R: ACACGCACTCGCACTACTCG	Chr.4	图位克隆	SSR
AP003312-0	F: CGTTCAGGAAAGTAGTGCAAGCC	Chr.1	图位克隆	InDel
AP003312-0	R: TCCAATTGAGTATGTGTGCTCA	Chr.1	图位克隆	InDel
AP003312-1	F: TGATGATACATGGGTCACCAGT	Chr.1	图位克隆	InDel
AP003312-1	R: GGTACTCGACTACTCTGCCC	Chr.1	图位克隆	InDel
OS01T0356900-RT	F: CACCGTTTTTCGAAGGATACTG	Os01g0356900	RT-PCR	/
OS01T0356900-RT	R: TCAATGTGGTTAAAGCATGAGC	Os01g0356900	RT-PCR	/
01G0356951-RT	F: ATCGCTATCTCGCCGAGTC	Os01g0356951	RT-PCR	/
01G0356951-RT	R: CAGCAAGTCTCCACAGTCCA	Os01g0356951	RT-PCR	/
OS01T0357100-RT	F: CGACGAACTTGTGAACCATTTT	Os01g0357100	RT-PCR	/
OS01T0357100-RT	R: CCAACACCGCAATTAACTGATA	Os01g0357100	RT-PCR	/
OS01T0357200-RT	F: TTTCGACGAGGAGGTATTCATC	Os01g0357200	RT-PCR	/
OS01T0357200-RT	R: CTCCCCTTGATCTTCAATTTGC	Os01g0357200	RT-PCR	/

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Design and exploration of epigenetic comprehensive experiments

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