Hereditas(Beijing) ›› 2021, Vol. 43 ›› Issue (7): 694-703.doi: 10.16288/j.yczz.20-423
• Technique and Method • Previous Articles Next Articles
A method for constructing GLP-1 overexpression intestinal organoids
Zhiyang Zeng1(
), Jiawei Lu1, Xiya Cao1, Xinyue Wang2,3, Dali Li1()
- 1. Shanghai Key Laboratory of Regulatory Biology, School of Life Sciences, East China Normal University, Shanghai 200241, China
2. School of Medicine, Anhui University of Science and Technology, Huainan 232000, China
3. Department of Gastroenterology, South Campus of Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 201499, China
-
Received:2021-03-01Revised:2021-04-14Online:2021-07-20Published:2021-07-20 -
Contact:Li Dali E-mail:52161300029@stu.ecnu.edu.cn;dlli@bio.ecnu.edu.cn -
Supported by:Supported by the National Key R&D Program of China No(2019YFA0110802)
Cite this article
Zhiyang Zeng, Jiawei Lu, Xiya Cao, Xinyue Wang, Dali Li. A method for constructing GLP-1 overexpression intestinal organoids[J]. Hereditas(Beijing), 2021, 43(7): 694-703.
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Fig. 1
Packaging and validation of GLP-1/Fc lentivirus"
Fig. 1
Fig. 2
Optimization of conditions for organoids’lentivirus infection"
Fig. 2
Fig. 3
Construction of GLP-1/Fc overexpression organoids"
Fig. 3
Fig. 4
GLP-1/Fc can quickly restore blood glucose balance in WT and diabetic mice in glucose tolerance test"
Fig. 4
Fig. 5
GFP-organoids were orthotopically transplanted into the small intestine of mice"
Fig. 5
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