陆地棉皱缩叶突变体基因wr3的初步定位

doi:10.3724/SP.J.1005.2014.1256

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HEREDITAS(Beijing) ›› 2014, Vol. 36 ›› Issue (12): 1256-1260.doi: 10.3724/SP.J.1005.2014.1256

• Research Articles • Previous Articles Next Articles

Mapping of a new wrinkled leaf (wr3) gene in upland cotton

Fengli Li¹, Jiachun Di², Liang Zhao², Xusheng Chen²

1. Agronomy College of Nanjing Agricultural University, Nanjing 210095, China;
2. Key Laboratory of Cotton and Rape in the Lower Reaches of the Yangtze River, Ministry of Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China

Received:2014-06-04 Online:2014-12-20 Published:2014-12-20

Abstract

Abstract:

The phenotype of a wrinkled leaf mutant in upland cotton newly found was different from other analogous mutants reported previously. Genetic analysis indicates that the mutation is controlled by a single recessive gene wr3. In this paper, an F2 mapping population derived from the mutation inbred line L037 crossing with Hai7124 (Gossypium babardense) was used. A total of 12 pairs of polymorphic primers were selected from 234 pairs of SSR primers obtained before, which covered 26 pairs of cotton chromosomes at regular intervals. Genetic mapping of the F2 population showed that gene wr3 was linked to the primer BNL3279 on Chr.21 with the distance of 28.7 cM. Further screening of other primers on Chr.21 showed that gene wr3 was flanked by the primers NAU3740 and cgr5428, with genetic distance of 4.8 cM and 10.4 cM, respectively.

Key words: Gossypium hirsutum, wrinkled leaf mutant, gene mapping, SSR, bulked segregation analysis

Fengli Li, Jiachun Di, Liang Zhao, Xusheng Chen. Mapping of a new wrinkled leaf (wr3) gene in upland cotton[J]. HEREDITAS(Beijing), 2014, 36(12): 1256-1260.

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Mapping of a new wrinkled leaf (wr3) gene in upland cotton

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