水稻苗期耐盐突变体的遗传分析及基因定位

doi:10.3724/SP.J.1005.2013.01101

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HEREDITAS ›› 2013, Vol. 35 ›› Issue (9): 1101-1105.doi: 10.3724/SP.J.1005.2013.01101

Genetic analysis and gene mapping for a salt tolerant mutant at seedling stage in rice

WANG Bin^1,2, LIU Ting-Ting^1,3, ZHANG Shu-Jun^1,2, LAN Tao^1,3, GUAN Hua-Zhong^1,3, ZHOU Yuan-Chang^1,3, WU Wei-Ren^1,3

1. Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
2. College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
3. College of Crop Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China

Received:2013-04-15 Revised:2013-05-28 Online:2013-09-20 Published:2013-09-25
Contact: Weiren Wu E-mail:wuwr@fafu.edu.cn

Abstract

Abstract:

A salt tolerant mutant at seedling stage was obtained from an M2 population of radiation mutagenesis of an indica rice cultivar R401. The mutant seedlings could survive under the treatment of sodium chloride solution at the concentration of 150 mmol/ L, while the wild-type control seedlings withered and died. An F2 population was developed from a cross between a japonica cultivar Nipponbare and the salt tolerant mutant. By investigating the performance of the F2 population under the stress of 150 mmol/ L NaCl solution, we found that the mutant phenotype was caused by the recessive mutation of a single gene, temporarily designated SST(t). Bulked segregant analysis (BSA) based on the F2 mapping population revealed that SST(t) is located on chromosome 6. By analyzing 137 typical salt-tolerant F2 plants using molecular markers, SST(t) was mapped in a 2.3 cM (or 406 kb) interval between InDel markers ID26847 and ID27253, with genetic distances of 1.2 cM and 1.1 cM to the two markers, respectively.

Key words: gene mapping, seedling, genetic analysis, rice, salt tolerance

WANG Bin LIU Ting-Ting ZHANG Shu-Jun LAN Tao GUAN Hua-Zhong ZHOU Yuan-Chang WU Wei-Ren. Genetic analysis and gene mapping for a salt tolerant mutant at seedling stage in rice[J]. HEREDITAS, 2013, 35(9): 1101-1105.

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Genetic analysis and gene mapping for a salt tolerant mutant at seedling stage in rice

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