Ssu72磷酸酶缺失导致减数第二次分裂过程中纺锤体交叉

doi:10.16288/j.yczz.24-047

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Hereditas(Beijing) ›› 2024, Vol. 46 ›› Issue (6): 502-508.doi: 10.16288/j.yczz.24-047

• Research Article • Previous Articles

Ssu72 phosphatase deficiency leads to spindle crossing during the second meiotic division process

Yan Jingliang¹^,²(), Ma Lingling¹^,², Watanabe Yoshinori¹^,²()

1. Science Center for Future Foods, Jiangnan University, Wuxi 214122
2. School of Biotechnology, Jiangnan University, Wuxi 214122

Received:2024-02-22 Revised:2024-04-27 Online:2024-06-20 Published:2024-05-08

Abstract

Abstract:

Ssu72 is a component of the yeast cleavage/polyadenylation factor (CPF) complex, which catalyzes the dephosphorylation of the C-terminal domain (CTD) of RNA polymerase II at S5-P and S7-P. It has been shown that Ssu72 phosphatase is involved in regulating chromosome cohesion during mitosis. To further clarify whether Ssu72 phosphatase affects chromosome separation during meiotic division in Schizosaccharomyces pombe, we utilized green fluorescent protein (GFP) to label centromeres and red fluorescent protein to label microtubule protein Atb2. The entire meiotic chromosome separation process of ssu72∆ cells was observed in real-time under fluorescence microscope. It was found that two spindles of ssu72∆ cells crossed during the metaphase and anaphase of the second meiotic division, and this spindle crossing led to a new type of spore defect distribution pattern. The results of this study can provide important reference significance for studying the roles of phosphatase Ssu72 in higher organisms.

Key words: fission yeast, meiosis, Ssu72, spindle crossing

Yan Jingliang, Ma Lingling, Watanabe Yoshinori. Ssu72 phosphatase deficiency leads to spindle crossing during the second meiotic division process[J]. Hereditas(Beijing), 2024, 46(6): 502-508.

Figures/Tables 6

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References 23

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菌株名称	基因型
Y355	h^- leu1-32 imr1L-GFP mes1-B44
Y491	h⁺ mes1-B44
YA1	h^- leu1-32 imr1L-GFP ∆ssu72::hyg mes1-B44
YA2	h⁺ ∆ssu72::hyg mes1-B44
L352	h^- leu1-32 imr1L-GFP
Y17	h⁺
YA53	h^- leu1-32 imr1L-GFP ∆ssu72::hyg
YA54	h⁺ ∆ssu72::hyg
YA59	h^- leu1-32 imr1L-GFP z::nat-Padh13-mCherry-atb2 ∆ssu72::hyg
YA60	h⁺ z::nat-Padh13-mCherry-atb2 ∆ssu72::hyg

Ssu72 phosphatase deficiency leads to spindle crossing during the second meiotic division process

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