着丝粒蛋白Fta2磷酸化对减数分裂的影响

doi:10.16288/j.yczz.24-038

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Hereditas(Beijing) ›› 2024, Vol. 46 ›› Issue (7): 552-559.doi: 10.16288/j.yczz.24-038

• Research Article • Previous Articles Next Articles

The effect of centromere protein Fta2 phosphorylation during meiosis

Zihan Ni¹^,²(), Yu Min¹^,², Lingling Ma¹^,², Yoshinori Watanabe¹^,²()

1. Science Center for Future Foods, Jiangnan University, Wuxi 214122, China
2. Biotechnology School, Jiangnan University,Wuxi 214122,China

Received:2024-03-14 Revised:2024-05-06 Online:2024-07-20 Published:2024-05-22

Abstract

Abstract:

During meiosis, defects in cohesin localization within the centromere region can result in various diseases. Accurate cohesin localization depends on the Mis4-Ssl3 loading complex. Although it is known that cohesin completes the loading process with the help of the loading complex, the mechanisms underlying its localization in the centromere region remain unclear. Previous studies suggest cohesin localization in the centromere is mediated by phosphorylation of centromeric proteins. In this study, we focused on the Fta2 protein, a component of the Sim4 centromere protein complex. Using bioinformatics methods, potential phosphorylation sites were identified, and fta2-9A and fta2-9D mutants were constructed in Schizosaccharomyces pombe. The phenotypes of these mutants were characterized through testing thiabendazole (TBZ) sensitivity and fluorescent microscopy localization. Results indicated that Fta2 phosphorylation did not impact mitosis but affected chromosome segregation during meiosis. This study suggests that Fta2 phosphorylation is vital for meiosis and may be related to the specific localization of cohesin during this process.

Key words: meiosis, cohesin, Fta2, phosphorylation, loading complex

Zihan Ni, Yu Min, Lingling Ma, Yoshinori Watanabe. The effect of centromere protein Fta2 phosphorylation during meiosis[J]. Hereditas(Beijing), 2024, 46(7): 552-559.

Figures/Tables 6

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菌株名称	基因型
Y17	h⁺
Y355	h^- leu1 imr1L-GFP mes1-B44
Y491	h⁺ mes1^-B44
N1	h^- leu1 imr1L-GFP mes1-B44 fta2-nat
N2	h⁺ mes1-B44 Fta2-nat
N3	h^- leu1 imr1L-GFP mes1-B44 rec8-2A-kan rec12::hyg
N4	h⁺ mes1-B44 rec8-2A-kan rec12::hyg
N5	h^- leu1 imr1L-GFP mes1-B44 rec8-2A-kan rec12::hyg fta2-nat
N6	h^- leu1 imr1L-GFP mes1-B44 rec8-2A-kan rec12::hyg fta2-nat-9A
N7	h^- leu1 imr1L-GFP mes1-B44 rec8-2A-kan rec12::hyg fta2-nat-9D
N8	h⁺ mes1-B44 rec8-2A-kan rec12::hyg fta2-nat
N9	h⁺ mes1-B44 rec8-2A-kan rec12::hyg fta2-nat-9A
N10	h⁺ mes1-B44 rec8-2A-kan rec12::hyg fta2-nat-9D
N11	h⁺ fta2-nat
N12	h⁺ fta2-nat-9A
N13	h⁺ fta2-nat-9D
N53	h⁺ swi6::kanr

CCAN	S. pombe	S. cerevisiae
C	Cnp3	Mif2
O	Mal2	Mcm21
P	Fta2	Ctf19
Q	Fta7	Okp1
U	Mis17	Ame1
H	Fta3	Mcm16
I	Mis6	Ctf3
K	Sim4	Mcm22
T	Cnp20	Cnn1
W	SPAC17G8.15 (new1)	Wip1
S	SPBC2D10.16	Mhf1
X	SPCC576.12c	Mhf2
N	Mis15	Chl4
L	Fta1	Iml3

The effect of centromere protein Fta2 phosphorylation during meiosis

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