斑马鱼ppp6r3调控性腺分化和配子发生的作用研究

doi:10.16288/j.yczz.25-093

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Hereditas(Beijing) ›› 2025, Vol. 47 ›› Issue (9): 1023-1031.doi: 10.16288/j.yczz.25-093

• Research Article • Previous Articles Next Articles

Role of ppp6r3 in zebrafish gonadal differentiation and gametogenesis

Yunhai Gao¹^,²(), Jiajie Deng², Xiao Xiao², Luyuan Pan³, Mudan He²(), Yunbin Zhang¹()

1. Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai 200031, China
2. Institute of Hydrobiology, Chinese Academy of Science, Wuhan 430070, China
3. China Zebrafish Resource Center, National Aquatic Biological Resource Center, Institute of Hydrobiology, Chinese Academy of Science, Wuhan 430070, China

Received:2025-04-02 Revised:2025-04-24 Online:2025-05-13 Published:2025-05-13
Contact: Mudan He, Yunbin Zhang E-mail:gaoyunhai2022@sibcb.ac.cn;hemudan@ihb.ac.cn;zhangyb@sibcb.ac.cn
Supported by:
National Key R&D Program of China(2022YFF0710500);National Key R&D Program of China(2024YFD2402300);STI 2030-Major Projects(2023ZD04069)

Abstract

Abstract:

The gonad differentiation and gametogenesis of fish is regulated by various factors. Protein phosphatase (PP) have the function of catalyzing the dephosphorylation of proteins in organisms. As a member of the protein phosphatase family, protein phosphatase type 6 (PP6) plays an important role in gonadal development and gametogenesis. The role of ppp6r3, which encodes the regulatory subunit 3 of protein phosphatase 6, in gonadal differentiation and gametogenesis is still unclear. In this study, two zebrafish ppp6r3 mutant lines were constructed using CRISPR/Cas9 technology. It was found that the absence of ppp6r3 leads to the development of only male zebrafish, and these mutants are incapable of fertilizing wild-type eggs. Further investigations revealed that in the testes of ppp6r3 mutants, the transition of spermatocytes to mature sperm was blocked, disrupting spermatogenesis. In summary, this study established a ppp6r3 mutant model, exhibiting defects in gonadal differentiation and gametogenesis, which provides a new model for further research on the mechanisms by which Ppp6r3 regulates germ cell proliferation, differentiation, and sex determination.

Key words: Ppp6r3, zebrafish, gonadal differentiation, gametogenesis

Yunhai Gao, Jiajie Deng, Xiao Xiao, Luyuan Pan, Mudan He, Yunbin Zhang. Role of ppp6r3 in zebrafish gonadal differentiation and gametogenesis[J]. Hereditas(Beijing), 2025, 47(9): 1023-1031.

Figures/Tables 6

Table 1

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References 19

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引物	序列(5′→3′)	用途
ppp6r3-gRNA-F	TGTAATACGACTCACTATAACGGATGGTTCATTCCAGGGGTTTTA GAGCTAGAAATAGC	gRNA扩增
gRNA-RP	AAAAAAAGCACCGACTCGGTGCCAC	gRNA扩增
ppp6r3-cas9-F	TGGAGGGATTAAGGTTGAGCT	靶点扩增和突变体筛选
ppp6r3-cas9-R	GTCCAAGACTCTGCCAGGTT	靶点扩增和突变体筛选
Ppp6r3-P1	CCTCGTAAATCCTCATCAAAAGCTGTAGAGTTTCATCAGTAAACT	单分子荧光检测
Ppp6r3-P2	GGTTGAGCGGAGGCTCGTTCTGTAAAAATCATCCAGTAAACCGCC
Ppp6r3-P3	CCTCGTAAATCCTCATCAAATCAAGACTTTGCTGAAGAAACTGGC
Ppp6r3-P4	GCTCTGGTTTTCTCCCAATAAGTATAAATCATCCAGTAAACCGCC
Ppp6r3-P5	CCTCGTAAATCCTCATCAAAAGTCTTCCCTCTTCCTGAGGAACTC
Ppp6r3-P6	CTATGTGTTTGATCATCAGATCCACAAATCATCCAGTAAACCGCC
Ppp6r3-P7	CCTCGTAAATCCTCATCAAATCCTGAGCAGGAGGTCCATAATAGC
Ppp6r3-P8	GCTGCTGTGGCTCAATGCAGGTGAGAAATCATCCAGTAAACCGCC
Ppp6r3-P9	CCTCGTAAATCCTCATCAAACTTCATTTAGCCAATTTAATACATC
Ppp6r3-P10	TATCAACCAGCCGCTGTATAACTTTAAATCATCCAGTAAACCGCC

Role of ppp6r3 in zebrafish gonadal differentiation and gametogenesis

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