义乌小鲵肢体再生及其分子机制

doi:10.16288/j.yczz.24-326

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Hereditas(Beijing) ›› 2025, Vol. 47 ›› Issue (9): 1032-1041.doi: 10.16288/j.yczz.24-326

• Research Article • Previous Articles Next Articles

The molecular mechanism of limb regeneration of Hynobius yiwuensis

Hao Chen¹(), Rui Dong¹, Yanfeng Wang², Jingbei Xie¹, Zhaoxu Zhang¹, Rongquan Zheng¹^,³()

1. Provincial Key Laboratory of Wildlife Biotechnology Protection and Utilization, Zhejiang Normal University, Jinhua 321004, China
2. Zhejiang Province Yongkang Municipal Natural Resources and Planning Bureau, Yongkang 321300, China
3. Xingzhi College, Zhejiang Normal University, Jinhua 321000, China

Received:2024-11-11 Revised:2025-01-17 Online:2025-05-07 Published:2025-05-07
Contact: Rongquan Zheng E-mail:898148121@qq.com;zhengrq@zjnu.cn
Supported by:
National Natural Science Foundation of China(32370454);Key R&D Program of Zhejiang Province(2021C02044);Rescue and Conservation Project for Rare and Endangered Wildlife (Hynobius yiwuensis) Funded by the Yiwu Branch of the Jinhua Ecological Environment Bureau(ZXZJZC2022331GK);Wildlife Resource Background Survey Project of Yongkang City

Abstract

Abstract:

Hynobius yiwuensis is a vulnerable species endemic to China, restricted to specific hilly regions in Zhejiang Province. This study employed stereomicroscopy to examine the limb regeneration process in H. yiwuensis and utilized transcriptome sequencing to analyze differentially expressed genes. The results indicate that H. yiwuensis possesses strong regenerative capabilities, with the regeneration process comprising four stages: wound healing, tissue dissolution and dedifferentiation, blastema formation, and morphogenesis followed by redifferentiation. Transcriptome analysis identified numerous differentially expressed genes during limb regeneration, exhibiting distinct expression patterns at various time points post-amputation. Key differentially expressed genes were identified, including IL10, associated with cellular immunity and inflammation; TGFβ3, involved in early muscle tissue regeneration; and MMPs, implicated in tissue remodeling. qRT-PCR validation of selected differentially expressed genes confirmed the reliability of the transcriptome sequencing data. Preliminary findings suggest that H. yiwuensis regulates limb regeneration and promotes scar-free tissue repair through signaling pathways such as Wnt/β-catenin, TGFβ, and BMP.

Key words: Hynobius yiwuensis, limb regeneration, transcriptome, differentially expressed genes

Hao Chen, Rui Dong, Yanfeng Wang, Jingbei Xie, Zhaoxu Zhang, Rongquan Zheng. The molecular mechanism of limb regeneration of Hynobius yiwuensis[J]. Hereditas(Beijing), 2025, 47(9): 1032-1041.

Figures/Tables 6

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