遗传 ›› 2009, Vol. 31 ›› Issue (9): 921-935.doi: 10.3724/SP.J.1005.2009.00921

• 研究报告 • 上一篇    下一篇

广西4个少数民族17个Y-STR基因座的多态性分析

冯冬亮1;刘长晖2;梁祚仁3;刘超2

  

  1. 1. 中山大学中山医学院法医学系, 广州 510080;
    2. 广州市刑事科学技术研究所, 广州 510030;
    3. 右江民族医学院, 广西百色 533000

  • 收稿日期:2009-03-09 修回日期:2009-05-04 出版日期:2009-09-10 发布日期:2009-09-10

Genetic polymorphism of 17 Y-STR loci in four minority populations in Guangxi of China

FENG Dong-Liang1;LIU Chang-Hui2;LIANG Zuo-Ren3;LIU Chao2   

  1. 1. Department of Forensic Biology, Sun Yat-Sen University, Guangzhou 510080, China;
    2. Guangzhou Institute of Criminal Science and Technology, Guangzhou 510030, China; 3. Youjiang Medical College for Nationalities, Baise, Guangxi 533000, China
  • Received:2009-03-09 Revised:2009-05-04 Published:2009-09-10 Online:2009-09-10

PDF (PC)

2408

被引次数

33

摘要：

应用Yfiler复合扩增试剂盒及基因分型技术, 调查了广西瑶、彝、京、壮4个少数民族人群17个Y-STR基因座的多态性分布, 计算等位基因频率和单体型多样性, 并结合国内其他4个群体相应基因座的遗传学资料, 分析其遗传距离和聚类关系。瑶族100名无关男性个体中检出61种单体型, 单体型多样性为0.9784; 彝族105名无关男性个体中检出67种单体型, 单体型多样性为0.9866; 京族103名无关男性个体中检出79种单体型, 单体型多样性为0.9911; 壮族107名无关男性个体中检出91种单体型, 单体型多样性为0.9956。在4个少数民族中, 京族和壮族的遗传距离最小(0.0391), 彝族和瑶族的遗传距离最大(0.3376)。结果表明, 该17个Y-STR基因座在广西4个少数民族人群中均具有较高的多态性, 在法医学、群体遗传学等方面有重要的应用价值。

关键词: 遗传多态性, STR, 遗传距离, Y染色体, 单体型

Abstract:

To investigate the genetic polymorphism of Y-chromosomal short tandem repeats (STR) loci in Jing, Yi, Yao, and Zhuang minority populations from Guangxi Province, China. 17 Y-STR loci were co-amplified using AmpFlSTR® Yfiler™ PCR Amplification Kit System, and the PCR products were analyzed by genetic analyzer. Cluster and phylogenic tree analyses were conducted to show the genetic distance among the populations. There were 61 different haplotypes in 100 unrelated Yao males, 67 in 105 unrelated Yi males, 79 in 103 unrelated Jing males, and 91 in 107 unrelated Zhuang males. The haplotype diversities of Jing, Yi, Yao and Zhuang were determined as 0.9784, 0.9866, 0.9911, and 0.9956, respectively. Among these 4 minority populations, the genetic distance between Jing and Zhuang was the smallest (0.0391), while the genetic distance between Yi and Yao was the largest (0.3376). The 17 Y-STR loci in the 4 minority populations from Guangxi Province revealed a highly polymorphic genetic distribution, which show a high potential for population genetics and forensic practice.

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