采用测序和单链构象多态(Single-strand conformation polymorphism, SSCP)的方法, 分析了PRKAB2 (Protein kinase, AMP-activated, beta 2 non-catalytic subunit)基因在5个优质肉鸡纯系和3个杂交配套系中的遗传分布、遗传变异及群体杂合性等群体遗传信息, 并分析了PRKAB2基因对屠宰性状及肉质性状的遗传效应。结果发现该基因的CDS区域非常保守, 仅在外显子1的406 bp处发生点突变, 由T→C。野生型A基因可显著提高活重、屠体重、腿肌重和腹脂重(P < 0.05), 同时也会提高肉的嫩度。

SSCP (Single-strand conformation polymorphism, SSCP) technique was applied in this study to analyze the population genetic information about genetic distribution, variation and heterozygosity of PRKAB2(Protein kinase, AMP-activated, beta 2 non-catalytic subunit) gene in five purelines and three crossbreds. The genetic effects of PRKAB2 gene on fiber density and partial carcass traits were also analyzed. Only one point mutation T→C at base position 406 located among the first exon of the whole CDS was found in the whole CDS.The wild-type allele A had significant positive effects on live-weight, carcass-weight, leg-muscle-weight and abdomen-fat-weight (P<0.05), and had positive effects on meat tenderness.