文章利用已建立的苏太猪F18大肠杆菌病抗性和敏感性资源家系群体作为实验材料, 分别选择8头35日龄左右生长性状基本一致的大肠杆菌 F18菌株抗性和敏感性断奶仔猪, 运用Real-time PCR方法检测BPI基因mRNA在断奶仔猪各个组织的分布情况, 并比较其在大肠杆菌 F18菌株抗性型和敏感型断奶仔猪个体间的差异表达水平, 为探讨该基因在免疫和抗大肠杆菌F18菌株感染中的作用提供依据。结果表明, 在对所有个体检测的11个组织中, BPI基因在心、肝、脾、肺、肾、胃、肌肉、胸腺、淋巴结中几乎不表达, 或表达量很低, 但在十二指肠和空肠中表达量很高。在十二指肠和空肠中, BPI基因在抗性组的表达量均显著高于敏感组的表达量(P<0.05)。由此表明, BPI基因对抗断奶仔猪肠道中大肠杆菌F18菌株的感染可能具有直接作用, 并且个体对大肠杆菌F18菌株的抗性可能与BPI基因在肠道中表达量上调有关。
Based on the established resource populations of Sutai pig, the expression of BPI gene was assayed by Real-time PCR to detect the tissue expression and analyze the differential expression between Escherichia coli F18-resistant and sensitive piglets. This study aimed at providing a theoretical foundation for further research on the role BPI gene in host immunity and resistance to E. coli F18. The results showed that the expression of BPI gene was extremely low or undetectable in tissues including heart, liver, spleen, lung, kidney, stomach, muscle, thymus, and lymph nodes, which was in a stark contrast to the significantly high levels in duodenum and jejunum. In the tissues of both jejunum and duodenum, the mRNA expression of BPI gene in resistant individuals was significant higher than that in the sensitive individuals (P<0.05). The results suggested that BPI gene was likely to be related to the intestinal infection caused by E. coli F18. It is possible that the increased expression of BPI gene in intestinal is in connection with the resistance to E. coli F18.
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