植物中存在许多多效性基因, 它们在调控植物的营养生长与生殖发育过程中起着关键性作用。文章在籼稻育种材料中发现了一个植株显著矮化且花器官明显变异的突变体ddf1(dwarf and deformed flower 1)。遗传分析表明, 该突变体由单基因隐性突变所致, 这说明该基因是一个同时控制营养生长和生殖发育的多效性基因, 暂命名为DDF1。为了定位该基因, 将ddf1杂合体与热带粳稻品种DZ60杂交, 建立了F2定位群体, 利用水稻RM系列微卫星标记, 通过混合分离分析(BSA)和小群体连锁分析, 将DDF1初步定位在水稻第6号染色体RM588和RM587标记之间, 与两标记的遗传距离分别为3.8 cM和2.4 cM。进一步利用已经公布的水稻基因组序列, 在初步定位的区间内开发新的SSR标记, 将DDF1定位在165 kb的区间内。该结果为克隆DDF1奠定了基础。
There are many pleiotropic genes playing key roles in regulating both vegetative growth and reproductive development in plants. A dwarf mutant of rice with deformed flowers, named as ddf1, was identified from indica rice breeding lines. Genetic analysis indicated that ddf1 was resulted from the recessive mutation of a single gene, temporarily named as DDF1. This result suggested that DDF1 is a pleiotropic gene, which controls both vegetative growth and reproductive development in rice. To map this gene, an F2 population was developed by crossing the ddf1 heterozygote with the tropical japonica rice variety DZ60. By means of bulked segregant analysis and small population-based linkage analysis using the published RM-series rice SSR markers, DDF1 was preliminarily mapped in a region between markers RM588 and RM587 on chromosome 6 with the ge-netic distances of 3.8 cM and 2.4 cM to the two markers, respectively. By developing new SSR markers in this interval ac-cording to the published rice genome sequence, we further mapped DDF1 in a 165 kb interval. The results will fa-cilitate cloning of DDF1.
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