研究报告

雷蒙德氏棉HSP70基因家族的进化分析及其同源基因在陆地棉中的表达分析

展开
  • 1. 浙江农林大学,亚热带森林培育国家重点实验室培育基地,临安 311300; 2. 浙江农林大学农业与食品科学学院,浙江省农产品品质改良技术研究重点实验室,临安 311300; 3. 中国农业科学院棉花研究所,棉花生物学国家重点实验室,安阳 45500
张毓婷,在读硕士研究生,专业方向:棉花遗传与分子育种。

收稿日期: 2014-03-11

  网络出版日期: 2014-09-20

基金资助

国家高技术研究发展计划项目(863计划)(编号:2011AA100202),国家自然科学基金项目(编号: 31200909),浙江省自然科学基金项目(编号: LQ12C06002)和棉花生物学国家重点实验室开放课题(编号:CB2013A02,CB2014B04)资助

Genome-wide analysis of HSP70 superfamily in Gossypium raimondii and the expression of orthologs in Gossypium hirsutum

Expand
  • 1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Lin’an 311300, China; 2. The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, School of Agriculture and Food Science, Zhejiang Agricultural and Forestry University, Lin’an 311300, China; 3.Institute of Cotton Research, Chinese Academy of Agricultural Sciences, State Key Laboratory of Cotton Biology, Anyang 455000, China

Received date: 2014-03-11

  Online published: 2014-09-20

摘要

热激蛋白70家族(HSP70)是一类在植物中高度保守的分子伴侣蛋白,在细胞中协助蛋白质正确折叠。文章利用隐马可链夫模型(HMM)在雷蒙德氏棉(Gossypium raimondii L.)全基因组范围内进行HSP70基因家族成员进化分析,共得到30个HSP70家族成员。利用生物信息学对雷蒙德氏棉HSP70基因的结构、染色体分布、基因倍增模式以及系统进化进行分析,结果表明,HSP70基因家族根据亚细胞定位结果可分为不同的基因亚家族,各亚家族中HSP70基因具有相对保守的基因结构;染色体片段重复和串联重复是雷蒙德氏棉HSP70基因家族扩增的主要方式。通过对不同物种的HSP70基因家族进行系统进化分析可知,HSP70亚组的分化发生在单细胞植物形成前,且细胞质型HSP70成员大量扩增。比较陆地棉棉纤维发育不同时期的深度测序表达谱,发现HSP70基因可能参与棉纤维的生长发育。本研究结果有助于了解棉属植物HSP70基因家族的功能,以期为深入研究棉纤维发育过程中的分子调控机理提供基础。

本文引用格式

张毓婷, 王敏华, 陈家栋, 戎均康, 丁明全 . 雷蒙德氏棉HSP70基因家族的进化分析及其同源基因在陆地棉中的表达分析[J]. 遗传, 2014 , 36(9) : 921 -933 . DOI: 10.3724/SP.J.1005.2014.0921

Abstract

Heat shock 70 (HSP70) proteins are highly conserved molecular chaperones widely existed in the plant kingdom which are involved in cellular protein folding process. In this study, comprehensive evolutionary analyses of the Gossypium raimondii HSP70 gene family members are conducted and 30 HSP70 genes are identified. The gene structure, chromosome distribution, gene duplication and phylogenic evolution of this family are further analyzed. The results reveal that HSP70 family genes can be clustered into several major subgroups based on their sub-cellular locations, and the gene structures are relatively conserved in each subgroup. Both tandem duplications and chromosome segmental duplications are found to contribute to the expansion of HSP70 gene family. Evolutionary analysis of HSP70s in diverse species reveals that the differentiation of HSP70 subgroups occurred before the multi-cell and single-cell differentiation, with the cytoplasmic HSP70s multiple amplified. The expression pattern of HSP70 genes under series of fiber development stages indicates that many HSP70 genes may participate in fiber development processes including fiber initiation and elongation. This study provides the complete profiles of cotton HSP70 family genes for future study on their functions related to the molecular mechanisms of fiber development.

参考文献

[1] ZY, Kohel RJ, Song GL, Cho J, Alabady M, Yu J, Koo P, Chu J, Yu SX, Wilkins TA, Zhu YX,Yu JZ. Gene-rich islands for fiber development in the cotton genome. Genomics , 2008, 92(3): 173-183.
[2] DL, Turley RB, Triplett BA, Meredith WR. Com-parison of protein profiles during cotton ( Gossypium hirsutum L.) fiber cell development with partial sequences of two proteins. J Agric Food Chem , 1996, 44(12): 4022-4027.
[3] 汤文开, 谭新, 龚路路, 李学宝. 棉纤维发育及其相关基因表达调控研究进展. 植物学通报, 2007, 24(2): 127-133.
[4] KB, Wang ZW, Li FG, Ye WW, Wang JY, Song GL, Yue Z, Cong L, Shang HH, Zhu SL, Li Q, Yuan YL, Lu CR, Wei HL, Gou CY, Zheng ZQ, Yin Y, Zhang XY, Liu K, Wang B, Song C, Shi N, Kohe RJ, Percy RG, Yu JZ, Zhu YX, Wang J, Yu SX. The draft genome of a diploid cotton Gossypium raimondii. Nature Genetics , 2012, 44(10): 1098-1103.
[5] LF, Pierce GJ, Bowers JE, Estill JC, Compton RO, Rainville LK, Kim C, Lemke C, Rong JK, Tang HB, Wang XY, Braidotti M, Chen AH, Chicola K, Collura K, Epps E, Golser W, Grover C, Ingles J, Karunakaran S, Kudrna D, Olive J, Tabassum N, Um E, Wissotski M, Yu Y, Zuccolo A, ur Rahman M, Peterson DG, Wing RA, Wendel JF, Paterson AH. A draft physical map of a D-genome cotton species ( Gossypium raimondii ). BMC Genomics , 2010, 11(1): 395.
[6] AH, Wendel JF, Gundlach H, Guo H, Jenkins J, Jin D, Llewellyn D, Showmaker KC, Shu S, Udall J, Yoo MJ, Byers R, Chen W, Doron-Faigenboim A, Duke MV, Gong L, Grimwood J, Grover C, Grupp K, Hu G, Lee TH, Li J, Lin L, Liu T, Marler BS, Page JT, Roberts AW, Romanel E, Sanders WS, Szadkowski E, Tan X, Tang H, Xu C, Wang J, Wang Z, Zhang D, Zhang L, Ashrafi H, Bedon F, Bowers JE, Brubaker CL, Chee PW, Das S, Gingle AR, Haigler CH, Harker D, Hoffmann LV, Hovav R, Jones DC, Lemke C, Mansoor S, ur Rahman M, Rainville LN, Rambani A, Reddy UK, Rong JK, Saranga Y, Scheffler BE, Scheffler JA, Stelly DM, Triplett BA, Van Deynze A, Vaslin MF, Waghmare VN, Walford SA, Wright RJ, Zaki EA, Zhang T, Dennis ES, Mayer KF, Peterson DG, Rokhsar DS, Wang X, Schmutz J. Repeated polyploidization of Gossypium genomes and the evolution of spinnable cotton fibres. Nature , 2012, 492(7429): 423-427.
[7] AT, Goldberg MD, Lund PA. Chaperones and protein folding in the archaea. Biochem Soc Trans , 2009, 37(Pt 1): 46-51.
[8] DA, Lindquist S. The function of heat shock proteins in stress tolerance: degradation and reactivation of damaged proteins. Annu Rev Biochem , 1993, 27(1): 437-496.
[9] JG, Tsokos GC. Heat shock protein 70 kDa: molecular biology, biochemistry, and physiology. Pharmacol Ther , 1998, 80(2): 183-201.
[10] B, Weissman J, Horwich A. Molecular chaperones and protein quality control. Cell , 2006, 125(3): 443-451.
[11] Z, Broadley SA, Shomura Y, Bracher A, Hartl FU. Molecular chaperones of the Hsp110 family act as nucleotide exchange factors of Hsp70s. EMBO J , 2006, 25(11): 2519-2528.
[12] QL, Hendrickson WA. Insights into Hsp70 chaperone activity from a crystal structure of the yeast Hsp110 Sse1. Cell , 2007, 131(1): 106-120.
[13] BL, Wang JS, Liu HC, Chen RW, Meyer Y, Barakat A, Delseny M. Genomic analysis of the Hsp70 superfamily in Arabidopsis thaliana . Cell Stress Chaperones , 2001, 6(3): 201-208.
[14] CL, Li QB. The organization and evolution of the spinach stress 70 molecular chaperone gene family. Plant Cell , 1998, 10(4): 539-556.
[15] NK, Kundnani P, Grover A. Functional analysis of Hsp70 superfamily proteins of rice ( Oryza sativa ). Cell Stress Chaperones , 2013, 18(4): 427-437.
[16] DY, Kaplan F, Guy CL. Plant Hsp70 molecular chaperones: protein structure, gene family, expression and function. Physiol Plant , 2002, 113(4): 443-451.
[17] LD, Cagna G, Stuttmann J, Wirthmüller L, Betsuyaku S, Witte CP, Bhat R, Pochon N, Colby T, Parker JE. Int-eraction between SGT1 and cytosolic/nuclear HSC70 chaperones regulates Arabidopsis immune responses. Plant Cell , 2007, 19(12): 4061-4076.
[18] 张天真, 贺亚军, 郭旺珍. 棉花纤维伸长发育期的基因表达分析. 作物学报,
文章导航

/