直立密穗基因<em>DEP2-1388</em>的遗传分析及在杂交稻中的育种利用

胡运高, 郭连安, 杨国涛, 钦鹏, 范存留, 彭友林, 严维, 何航, 李仕贵

doi:10.16288/j.yczz.15-158

遗传 >

2016 , Vol. 38 >Issue 1: 72 - 81

DOI: https://doi.org/10.16288/j.yczz.15-158

研究报告

直立密穗基因DEP2-1388的遗传分析及在杂交稻中的育种利用

展开

1. 四川农业大学水稻研究所,成都 611130;
2. 西南科技大学水稻研究所,绵阳 621010;
3. 北京大学生命科学院,北京 100871

胡运高,博士,研究员,专业方向：作物遗传育种。

收稿日期: 2015-06-24

修回日期: 2015-08-12

网络出版日期: 2016-01-20

基金资助

国家自然科学基金(编号：31471475)、国家科技支撑计划“长江上游优质抗病种质创制与新品种选育”(编号：2014BAD01B03)、四川省生物质资源利用与改性工程技术中心开展开放基金项目和杂交水稻国家重点实验室开放基金项目(编号：2014KF01)资助

收起

Genetic analysis of dense and erect panicle 2 allele DEP2-1388 and its application in hybrid rice breeding

Expand

1. Rice Research Institute of Sichuan Agricultural University, Chengdu 611130, China;
2. Rice Research Institute of Southwest University of Science and Technology, Mianyang 621010, China;
3. College of Life Sciences, Peking University, Beijing 100871, China

Received date: 2015-06-24

Revised date: 2015-08-12

Online published: 2016-01-20

Supported by

[Project Supported by the National Natural Science Foundation of China (No; 31471475), National Key Technology Support Program ‘Germplasm Creation and New Variety Breeding of High Quality and Disease Resistance in Upper Yangtze River’ (No; 2014BAD01B03), the Open Fund of Engineering Research Center for Biomass Resource Utilizaiton and Modification of Sichuan Province, and the Open Research Fund of State Key Laboratory of Hybrid Rice (Hunan Hybrid Rice Research Center) (No; 2014KF01)]

Fold

摘要

通过EMS诱变籼型重穗恢复系蜀恢498,获得一个直立穗突变体R1338。与野生型相比,突变体表现为植株变矮、穗直立、穗长变短、一次枝梗变短、着粒密度增加、穗部抗弯曲力极显著增强、籽粒增宽增厚、粒长变短。组织细胞学分析发现,穗颈节直径、纤维素含量和木质素含量在穗部抗弯曲上发挥了重要的作用。遗传分析表明该直立穗表型受一对半显性核基因DEP2控制,通过重测序以及MutMap方法定位发现,在R1338突变体中,DEP2第7外显子有一个A到G的碱基置换,导致第928个精氨酸(AGG)被置换成甘氨酸(GGG),推测R1338直立穗性状可能由DEP2中该SNP导致。用R1338、野生型与不同穗型不育系分别配组,R1338与弯曲穗不育系所配组合穗部表现半直立,且保持较高的结实率和杂种优势,与DEP1直立穗不育系配组表现为基因累加效应的直立穗。本研究还讨论了直立穗突变体R1338在杂交水稻育种中的利用价值。

关键词： 水稻; 直立穗; 基因定位; 群体密度; 育种应用

本文引用格式

胡运高, 郭连安, 杨国涛, 钦鹏, 范存留, 彭友林, 严维, 何航, 李仕贵 . 直立密穗基因DEP2-1388的遗传分析及在杂交稻中的育种利用[J]. 遗传, 2016 , 38(1) : 72 -81 . DOI: 10.16288/j.yczz.15-158

Abstract

Using ethyl methanesulfonate (EMS) mutagenesis, we isolated an erect panicle mutant, R1338, from indica heavy-panicle restorer Shuhui498. Compared with wild type control, the mutant displayed dwarfism, erect and short panicle, short primary panicle branch, increased grain density, short grain length and increased grain thickness. In addition, the erect panicle architecture of R1388 resulted in significant decreased bending moment and increased resistance to panicle bending. Histocytological analysis indicated that the diameter of uppermost internode, cellulose content and lignin content play important roles in resistance to panicle bending. Genetic analysis revealed that the mutant phenotype was controlled by a semi-dominant nuclear gene. With resequencing and MutMap analysis strategy, we found that one SNP from A to G at the seventh exon of DEP2 resulted in the 928^th amino acid substitution from arginine (AGG) to glycine (GGG) in R1338 mutant. Considering the phenotype of other dep2 mutants, the phenotype of R1338 was likely to be caused by the SNP in DEP2. The mutant R1338 and wild type were crossed with several sterile lines which respectively had different panicle types, the combinations generated from R1338 and curve panicle sterile lines showed semi-erect panicle, higher seed setting percentage and heterosis, and the combinations generated from R1388 and erect panicle sterile line with DEP1 showed erect panicle by gene additive effect. Moreover, the combinations with semi-erect panicle had superior light transmittance and stronger light intensity, which improved efficiency of light utilization to intermediate and subjacent leaves compared to the combinations with curved panicle. This study provides a good strategy to solve the problem of population density in three-line hybrid rice.

Key words： rice; erect panicle; gene mapping; population density; breeding application

参考文献

[1] Yuan LP. Hybrid rice breeding for super high yield. Hybrid Rice, 1997, 12(6): 1-6.
袁隆平. 杂交水稻超高产育种. 杂交水稻, 1997, 12(6): 1-6.
[2] Xu ZJ, Chen WF, Zhou HF, Zhang BL, Yang SR. The physiological and ecological characteristics of erect panicle rice population and its utilization prospect. Chinese Science Bulletin , 1996, 41(12): 1122-1126.
徐正进, 陈温福, 周洪飞, 张龙步, 杨守仁. 直立穗型水稻群体生理生态特性及其利用前景. 科学通报, 1996, 41(12): 1122-1126.
[3] Chen WF, Xu ZJ, Zhang LB. Physiological Basis Rice Breeding for Super High Yield. Shenyang: Liaoning Science and Technology Press, 1995.
陈温福, 徐正进, 张龙步. 水稻超高产育种生理基础. 沈阳: 辽宁科学技术出版社, 1995.
[4] Huang XZ, Qian Q, Liu ZB, Sun HY, He SY, Luo D, Xia GM, Chu CC, Li JY, Fu XD. Natural variation at the DEP1 locus enhances grain yield in rice. Nat Genet , 2009, 41(4): 494-497.
[5] Zhu KM, Tang D, Yan CJ, Chi ZC, Yu HX, Chen JM, Liang JS, Gu MH, Cheng ZK. ERECT PANICLE2 encodes a novel protein that regulates panicle erectness in Indica Rice . Genetics , 2010, 184(2): 343-350.
[6] Li F, Liu WB, Tang JY, Chen JF, Tong HN, Hu B, Li CL, Fang J, Chen MS, Chu CC. Rice DENSE AND ERECT PANICLE 2 is essential for determining panicle outgrowth and elongation. Cell Res , 2010, 20(7): 838-849.
[7] Abe Y, Mieda K, Ando T, Kono I, Yano M, Kitano H, Iwasaki Y. The SMALL AND ROUND SEED1 ( SRS1/DEP2 ) gene is involved in the regulation of seed size in rice. Genes Genet Syst , 2010, 85(5): 327-339.
[8] Piao RH, Jiang WZ, Ham TH, Choi MS, Qiao YL, Chu SH, Park JH, Woo MO, Jin ZX, An G, Lee J, Koh HJ. Map-based cloning of the ERECT PANICLE 3 gene in rice. Theor Appl Genet , 2009, 119(8): 1497-1506.
[9] Qiao YL, Piao RH, Shi JX, Lee SI, Jiang WZ, Kim BK, Lee J, Han LZ, Ma WB, Koh HJ. Fine mapping and candidate gene analysis of dense and erect panicle 3 , DEP3 , which confers high grain yield in rice ( Oryza sativa L.). Theor Appl Genet , 2011, 122(7): 1439-1449.
[10] Chen J, Gao H, Zheng XM, Jin MN, Weng JF, Ma J, Ren YL, Zhou KN, Wang Q, Wang J, Wang JL, Zhang X, Cheng ZJ, Wu CY, Wang HY, Wan JM. An evolutionarily conserved gene, FUWA , plays a role in determining panicle architecture, grain shape and grain weight in rice. Plant J , 2015, 83(3): 427-438.
[11] Duan PG, Rao YC, Zeng DL, Yang YL, Xu R, Zhang BL, Dong GJ, Qian Q, Li YH. SMALL GRAIN 1 , which encodes a mitogen-activated protein kinase kinase 4, influences grain size in rice. Plant J , 2014, 77(4): 547-557.
[12] Yang YH, Zhu Z, Zhang YD, Zhao QY, Zhou LH, Wang CL. Relationship between anatomic structure of the stem and lodging resistance of rice. Guihaia , 2012, 32(6): 834-839.
杨艳华, 朱镇, 张亚东, 赵庆勇, 周丽慧, 王才林. 水稻茎秆解剖结构与抗倒伏能力关系的研究. 广西植物, 2012, 32(6): 834-839.
[13] Xu ZJ, Chen WF, Han Y, Shao GJ, Zhang WZ, Ma DR. Classification of panicle type and its relationship with grain yield and quality of rice in liaoning province. Acta Agronomica Sinica , 2007, 33(9):1411-1418.
徐正进, 陈温福, 韩勇, 邵国军, 张文忠, 马殿荣. 辽宁水稻穗型分类及其与产量和品质的关系. 作物学报, 2007, 33(9): 1411-1418.
[14] Zhou Y, Zhu JY, Li ZY, Yi CD, Liu J, Zhang HG, Tang SZ, Gu MH, Liang GH. Deletion in a quantitative trait gene qPE9-1 associated with panicle erectness improves plant architecture during rice domestication. Genetics , 2009, 183(1): 315-324.
[15] Sun HY, Qian Q, Wu K, Luo JJ, Wang SS, Zhang CW, Ma YF, Liu Q, Huang XZ, Yuan QB, Han RX, Zhao M, Dong GJ, Guo LB, Zhu XD, Gou ZH, Wang W, Wu YJ, Lin HX, Fu XD. Heterotrimeric G proteins regulate nitrogen-use efficiency in rice. Nat Genet , 2014, 46(6): 652-656.
[16] Ookawa T, Hobo T, Yano M, Murata K, Ando T, Miura H, Asano K, Ochiai Y, Ikeda M, Nishitani R, Ebitani T, Ozaki H, Angeles ER, Hirasawa T, Matsuoka M. New approach for rice improvement using a pleiotropic QTL gene for lodging resistance and yield. Nat Commun , 2010, 1(8): 132.
[17] Hirano K, Okuno A, Hobo T, Ordonio R, Shinozaki Y, Asano K, Kitano H, Matsuoka M. Utilization of stiff culm trait of rice smos1 mutant for increased lodging resistance. PLoS One

Options

文章导航

〈

〉

www.chinagene.cn
备案号：京ICP备09063187号-4
总访问:,今日访问:,当前在线:

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献