CRISPR/Cas9系统在林木基因编辑中的应用
收稿日期: 2020-04-04
修回日期: 2020-05-25
网络出版日期: 2020-06-15
基金资助
青年人才托举工程编号(YESS20160121);江苏省高校“青蓝工程”优秀青年骨干教师项目和江苏省优势学科项目资助
Application of CRISPR/Cas9 mediated gene editing in trees
Received date: 2020-04-04
Revised date: 2020-05-25
Online published: 2020-06-15
Supported by
Supported by the Youth Elite Science Sponsorship Program by CAST No(YESS20160121);the Qing Lan Talent Support Program at Jiangsu Province, and the Priority Academic Program Development Program of Jiangsu Province
CRISPR/Cas9系统可以对目标基因进行精确定点编辑,是目前公认的最有发展潜力的基因编辑技术,并已在主要粮食及经济作物的精准育种方面发挥了重要作用。CRISPR/Cas9系统的出现也为林木基础研究和分子育种带来了新的途径。近年来CRISPR/Cas9系统在林木遗传研究中的应用越来越广泛,不仅实现了抗旱、抗病等林木新品种的培育,而且在调控木质素合成和缩短林木育种周期等方面也展现了巨大潜力。本文详细梳理了CRISPR/Cas9系统在林木基因功能验证及遗传改良中的研究进展,并对未来需要完善的相关问题和发展趋势进行了展望,以期为林木功能基因组研究和林木基因工程育种提供有益参考。
勘误：CRISPR/Cas9系统在林木基因编辑中的应用
关键词： CRISPR/Cas9; 基因编辑; 林木; 基因功能; 遗传改良
陈赢男, 陆静 . CRISPR/Cas9系统在林木基因编辑中的应用[J]. 遗传, 2020 , 42(7) : 657 -668 . DOI: 10.16288/j.yczz.20-092
The CRISPR/Cas9 system, which can induce precise modifications at a target gene, has been recognized as the most promising gene editing technology, and has played an important role in precision crop breeding. It also provides a new strategy for fundamental researches and molecular breeding of forest trees. Recently, CRISPR/Cas9-mediated gene editing has been applied more extensively in tree genetic studies. It has not only succeeded in developing new drought- resistance or disease-resistant cultivars, but also shows a great potential in regulating lignin biosynthesis and shortening the breeding cycle of forest trees. In this review, we summarize the application and advance of CRISPR/Cas9 in gene function identification and genetic improvement of forest plants. We also discuss the related problems and future perspectives. This review aims to provide a useful reference for tree functional genomics and genetic engineering breeding.
Key words： CRISPR/Cas9; gene editing; trees; gene function; genetic improvement
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