遗传 ›› 2020, Vol. 42 ›› Issue (7): 657-668.doi: 10.16288/j.yczz.20-092

• 综述 • 上一篇    下一篇

CRISPR/Cas9系统在林木基因编辑中的应用

陈赢男1,2, 陆静1,2   

  1. 1. 南京林业大学,林木遗传与生物技术省部共建教育部重点实验室,南京 210037
    2. 南京林业大学林学院,南方现代林业协同创新中心,南京 210037
  • 收稿日期:2020-04-04 修回日期:2020-05-25 出版日期:2020-07-20 发布日期:2020-06-15
  • 作者简介:陈赢男,博士,副教授,研究方向:林木遗传育种。E-mail: chenyingnan@njfu.edu.cn
  • 基金资助:
    青年人才托举工程编号(YESS20160121);江苏省高校“青蓝工程”优秀青年骨干教师项目和江苏省优势学科项目资助

Application of CRISPR/Cas9 mediated gene editing in trees

Chen Yingnan1,2, Lu Jing1,2   

  1. 1. Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing 210037, China
    2. Nanjing Forestry University, Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing 210037, China
  • Received:2020-04-04 Revised:2020-05-25 Online:2020-07-20 Published:2020-06-15
  • Supported by:
    Supported by the Youth Elite Science Sponsorship Program by CAST No(YESS20160121);the Qing Lan Talent Support Program at Jiangsu Province, and the Priority Academic Program Development Program of Jiangsu Province

摘要:

CRISPR/Cas9系统可以对目标基因进行精确定点编辑,是目前公认的最有发展潜力的基因编辑技术,并已在主要粮食及经济作物的精准育种方面发挥了重要作用。CRISPR/Cas9系统的出现也为林木基础研究和分子育种带来了新的途径。近年来CRISPR/Cas9系统在林木遗传研究中的应用越来越广泛,不仅实现了抗旱、抗病等林木新品种的培育,而且在调控木质素合成和缩短林木育种周期等方面也展现了巨大潜力。本文详细梳理了CRISPR/Cas9系统在林木基因功能验证及遗传改良中的研究进展,并对未来需要完善的相关问题和发展趋势进行了展望,以期为林木功能基因组研究和林木基因工程育种提供有益参考。

关键词: CRISPR/Cas9, 基因编辑, 林木, 基因功能, 遗传改良

Abstract:

The CRISPR/Cas9 system, which can induce precise modifications at a target gene, has been recognized as the most promising gene editing technology, and has played an important role in precision crop breeding. It also provides a new strategy for fundamental researches and molecular breeding of forest trees. Recently, CRISPR/Cas9-mediated gene editing has been applied more extensively in tree genetic studies. It has not only succeeded in developing new drought- resistance or disease-resistant cultivars, but also shows a great potential in regulating lignin biosynthesis and shortening the breeding cycle of forest trees. In this review, we summarize the application and advance of CRISPR/Cas9 in gene function identification and genetic improvement of forest plants. We also discuss the related problems and future perspectives. This review aims to provide a useful reference for tree functional genomics and genetic engineering breeding.

Key words: CRISPR/Cas9, gene editing, trees, gene function, genetic improvement