着丝粒蛋白Fta2磷酸化对减数分裂的影响
收稿日期: 2024-03-14
修回日期: 2024-05-06
网络出版日期: 2024-05-22
The effect of centromere protein Fta2 phosphorylation during meiosis
Received date: 2024-03-14
Revised date: 2024-05-06
Online published: 2024-05-22
在减数分裂过程中，黏连蛋白(cohesin)在着丝粒区域定位出现缺陷时会导致一系列疾病的产生。黏连蛋白的正确定位离不开装载复合体Mis4-Ssl3的参与，现已知黏连蛋白在装载复合体的帮助下完成装载过程，但是其如何在着丝粒区域定位仍不清楚。基于已有研究报道黏连蛋白在着丝粒的定位由着丝粒蛋白的磷酸化介导，本研究从Sim4着丝粒蛋白复合体组分Fta2蛋白着手，通过生物信息学手段寻找潜在的磷酸化位点，在裂殖酵母(Schizosaccharomyces pombe)中构建了fta2-9A和fta2-9D突变体，并通过噻苯咪唑(thiabendazole，TBZ)敏感度测试和荧光显微定位对其表型进行检测。结果显示，Fta2蛋白的磷酸化对有丝分裂没有影响，但对减数分裂染色体分离存在影响。本研究表明Fta2的磷酸化对减数分裂非常重要，很可能与减数分裂特有的黏连蛋白定位有关。
倪子涵, 闵羽, 马玲玲, 渡边嘉典 . 着丝粒蛋白Fta2磷酸化对减数分裂的影响[J]. 遗传, 2024 , 46(7) : 552 -559 . DOI: 10.16288/j.yczz.24-038
During meiosis, defects in cohesin localization within the centromere region can result in various diseases. Accurate cohesin localization depends on the Mis4-Ssl3 loading complex. Although it is known that cohesin completes the loading process with the help of the loading complex, the mechanisms underlying its localization in the centromere region remain unclear. Previous studies suggest cohesin localization in the centromere is mediated by phosphorylation of centromeric proteins. In this study, we focused on the Fta2 protein, a component of the Sim4 centromere protein complex. Using bioinformatics methods, potential phosphorylation sites were identified, and fta2-9A and fta2-9D mutants were constructed in Schizosaccharomyces pombe. The phenotypes of these mutants were characterized through testing thiabendazole (TBZ) sensitivity and fluorescent microscopy localization. Results indicated that Fta2 phosphorylation did not impact mitosis but affected chromosome segregation during meiosis. This study suggests that Fta2 phosphorylation is vital for meiosis and may be related to the specific localization of cohesin during this process.
Key words： meiosis; cohesin; Fta2; phosphorylation; loading complex
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