出生后小鼠心肌组织生长发育的转录组分析
朱晓聪和王圣楠并列第一作者。
收稿日期: 2024-11-13
修回日期: 2025-02-15
网络出版日期: 2025-04-21
基金资助
山东省现代农业产业技术体系驴创新团队建设专项基金(SDAIT-27)
Transcriptome analysis of postnatal mouse cardiac tissue growth and development
Received date: 2024-11-13
Revised date: 2025-02-15
Online published: 2025-04-21
Supported by
Donkey Innovation Team of Shandong Modern Agricultural Industry Technology System(SDAIT-27)
哺乳动物出生后的心脏功能与心肌细胞增殖和肥大密切相关，但涉及调控心肌细胞增殖和肥大的分子机制尚未完全阐明。为此，本研究对7日龄(P7)和3月龄(3m)的C57BL/6雌性小鼠心肌组织进行了表型测定和转录组测序分析，以探究小鼠心肌组织生长发育过程中心肌细胞的变化规律，并筛选影响心肌组织生长发育的关键候选基因。与7日龄相比，3月龄小鼠心脏重量和心肌细胞横截面积均显著增加(P<0.001)。通过转录组测序共鉴定到3,858个差异表达基因(differentially expressed genes，DEGs)，其中差异表达上调基因2,021个，差异表达下调基因1,837个。GO功能注释分析结果显示，差异表达基因显著富集于细胞周期、细胞分裂、心脏发育、细胞增殖等条目中。KEGG富集分析结果表明，差异表达基因显著富集于DNA复制、ECM-受体相互作用、细胞周期、代谢途径等信号通路。此外，通过定位筛选到Hey2、Foxm1、Igf1、Xirp2、Sfrp2、Egf、Fgfr2、Tbx20、Fgf1和Igf2等是与小鼠心肌组织生长发育相关的关键候选基因。qRT-PCR验证结果显示，与心肌组织生长发育相关的10个候选基因的表达趋势与RNA-seq结果相一致，说明测序结果的可靠性。本研究结果为进一步揭示小鼠心肌组织生长发育的分子机制提供了新的见解。
朱晓聪, 王圣楠, 蒋琳, 刘书琴 . 出生后小鼠心肌组织生长发育的转录组分析[J]. 遗传, 2025 , 47(10) : 1132 -1145 . DOI: 10.16288/j.yczz.24-328
Postnatal cardiac function in mammals is closely associated with cardiomyocyte proliferation and hypertrophy. However, the molecular mechanisms regulating cardiomyocyte proliferation and hypertrophy have not yet been fully elucidated. Therefore, phenotypic measurements and transcriptomic sequencing were performed on myocardial tissues from 7-day-old (P7) and 3-month-old (3m) female C57BL/6 mice to investigate changes in cardiomyocytes during growth and development and to identify key genes regulating myocardial growth and development. In comparison to 7-day-old mice, 3-month-old mice exhibited a significant increase in heart weight (P<0.001) and the cross-sectional area of cardiomyocytes (P<0.001). Transcriptome sequencing identified 3,858 differentially expressed genes (DEGs), including 2,021 up-regulated and 1,837 down-regulated genes. Gene Ontology (GO) functional annotation analysis demonstrated that the differentially expressed genes were significantly enriched in biological processes including cell cycle, cell division, cardiac morphogenesis and cellular proliferation. Significantly enriched KEGG pathways were identified, including those for DNA replication, ECM-receptor interaction, the cell cycle, metabolic pathways, and other signaling pathways. Furthermore, key candidate genes associated with myocardial tissue growth and development in mice, including Hey2, Foxm1, Igf1, Xirp2, Sfrp2, Egf, Fgfr2, Tbx20, Fgf1 and Igf2 were identified through screening. qRT-PCR validation results demonstrated that the expression trends of the 10 candidate genes related to myocardial growth and development were consistent with the RNA-seq results, confirming the reliability of the sequencing data. The findings of this study provide new insights into the molecular mechanisms underlying the growth and development of mouse myocardial tissue.
Key words： mice; myocardial tissue; growth and development; RNA-seq
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