砀山酥梨4CL基因家族的全基因组鉴定与分析

doi:10.16288/j.yczz.15-069

遗传 ›› 2015, Vol. 37 ›› Issue (7): 711-719.doi: 10.16288/j.yczz.15-069

砀山酥梨4CL基因家族的全基因组鉴定与分析

曹运鹏, 方志, 李姝妹, 闫冲冲, 丁庆庆, 程曦, 林毅, 郭宁, 蔡永萍

安徽农业大学生命科学学院,合肥 230036

收稿日期:2015-02-05 修回日期:2015-03-24 出版日期:2015-07-20 发布日期:2015-07-20
通讯作者: 蔡永萍, 教授, 博士, 研究方向：植物生物技术。E-mail: swkx12@ahau.edu.cn
郭宁, 副教授, 在读博士, 研究方向：遗传学、分子生物学等方面的教学和科研工作。E-mail: 81_ahau@ahau.edu.cn
作者简介:曹运鹏,硕士研究生,专业方向：砀山酥梨功能基因组学。E-mail: 499981806@qq.com
基金资助:
国家自然科学基金项目(编号：30771483, 31171944)和安徽省级大学生创新基金项目(编号：AH201410364038)资助

Genome-wide identification and analyses of 4CL gene families in Pyrus bretschneideri Rehd

Yunpeng Cao, Zhi Fang, Shumei Li, Chongchong Yan, Qingqing Ding, Xi Cheng, Yi Lin, Ning Guo, Yongping Cai

College of Chemistry and Life Sciences, Anhui Agricultural University, Hefei 230036, China

Received:2015-02-05 Revised:2015-03-24 Online:2015-07-20 Published:2015-07-20

摘要/Abstract

摘要： 4-香豆酸：辅酶A连接酶(4CL)基因是植物调控木质素代谢、参与类黄酮和其他次生代谢产物合成的关键基因之一,而木质素的合成及聚合在细胞壁沉积导致部分薄壁细胞次生壁加厚形成石细胞。为更好了解砀山酥梨中4CL基因的种类和数量,本文利用砀山酥梨基因组的氨基酸和cDNA数据库对4CL基因家族进行筛选,分析了砀山酥梨基因组中4CL基因的种类、进化关系、物理定位、以及基因结构和保守基序。结果显示在砀山酥梨基因组中发现并初步确定了29个4CL基因,通过基因的定位分析发现除了4、8、11、12号染色体上没有4CL基因之外,其他染色体上都存在4CL基因;并且在9、17号染色体上还存在着基因簇。通过基因结构和进化树之间的比较,进一步确定了基因结构和进化之间的相互联系。研究结果为砀山酥梨4CL基因功能的深入分析奠定了基础。

关键词: 砀山酥梨, 4CL, 基因结构, 进化

Abstract: 4-coumaric acid: coenzyme A ligase (4CL) gene is one of the key genes involved in the regulation of lignin metabolism and the synthesis of flavonoid and other secondary metabolites in plant, while the synthesized and polymerized lignin is deposited in cell walls and leads to thickening of secondary walls in some parenchyma cells and formation of stone cells. To better understand the variety and quantity of 4CL genes in Pyrus bretschneideri Rehd., we used the amino acid and cDNA databases of Pyrus bretschneideri Rehd. genome to screen 4CL gene family, and analyzed their classification, evolutionary relationships, physical location, gene structure and conserved motif. Our results showed that 29 4CL genes were identified and preliminarily characterized, and these 4CL genes were distributed in all chromosomes except chromosomes 4, 8, 11, 12 and clustered on chromosomes 9 and 17 through gene location analysis. The relationship between 4CL gene structure and evolution was further determined by comparing gene structure and phylogenetic tree. These findings provide a basis for further analysis of 4CL gene function in Pyrus bretschneideri Rehd.

Key words: Pyrus bretschneideri Rehd, 4CL, gene structure, phylogenetic

曹运鹏, 方志, 李姝妹, 闫冲冲, 丁庆庆, 程曦, 林毅, 郭宁, 蔡永萍. 砀山酥梨4CL基因家族的全基因组鉴定与分析[J]. 遗传, 2015, 37(7): 711-719.

Yunpeng Cao, Zhi Fang, Shumei Li, Chongchong Yan, Qingqing Ding, Xi Cheng, Yi Lin, Ning Guo, Yongping Cai. Genome-wide identification and analyses of 4CL gene families in Pyrus bretschneideri Rehd[J]. HEREDITAS(Beijing), 2015, 37(7): 711-719.

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砀山酥梨4CL基因家族的全基因组鉴定与分析

Genome-wide identification and analyses of 4CL gene families in Pyrus bretschneideri Rehd

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