遗传 ›› 2020, Vol. 42 ›› Issue (1): 32-44.doi: 10.16288/j.yczz.19-270

• 三维基因组专栏 • 上一篇    下一篇

细胞终末分化过程中三维基因组结构与功能调控的分子机制

杨科, 薛征, 吕湘()   

  1. 中国医学科学院基础医学研究所病理生理学系,医学分子生物学国家重点实验室,北京 100730
  • 收稿日期:2019-09-05 修回日期:2019-12-13 出版日期:2020-01-20 发布日期:2019-12-25
  • 通讯作者: 吕湘 E-mail:lvxiang@pumc.edu.cn
  • 作者简介:杨科,博士研究生,研究方向:造血调控与三维基因组。E-mail:yangk_92@163.com
  • 基金资助:
    国家重点研发计划干细胞及转化研究专项编号(2016YFA0100603);中国医学科学院医学与健康科技创新工程协同创新团队项目编号(2016-I2M-3-002);国家自然科学基金面上项目资助编号(81670108)

Molecular mechanism of the 3D genome structure and function regulation during cell terminal differentiation

Yang Ke, Xue Zheng, Lv Xiang()   

  1. State Key Laboratory of Medical Molecular Biology, Department of Pathophysiology, Institute of Basic Medical Sciences, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100730, China
  • Received:2019-09-05 Revised:2019-12-13 Online:2020-01-20 Published:2019-12-25
  • Contact: Lv Xiang E-mail:lvxiang@pumc.edu.cn
  • Supported by:
    Supported by the National Key Research and Development Program for Stem Cells and Transformation Research No(2016YFA0100603);Chinese Academy of Medical Sciences Medical and Health Science and Technology Innovation Engineering Collaborative Innovation Team Project No(2016-I2M-3-002);General Program of the National Natural Science Foundation of China No(81670108)

摘要:

真核细胞中的染色质DNA高度折叠形成复杂的三维结构,其空间组织方式对精准调控基因的表达和细胞发挥正常功能都起着重要的作用。细胞终末分化成熟过程中形态及基因表达谱常发生显著改变,同时伴随着明显的基因组三维结构变化。本文在简单介绍三维基因组多层次组织结构(染色质领域、A/B区室、拓扑相关结构域和成环构象等)基础上,重点综述了细胞终末分化过程中三维基因组结构变化与功能调控方面的研究进展,并探讨了当前三维基因组研究在解析细胞分化成熟过程时存在的问题和前景。

关键词: 三维基因组, A/B区室, 拓扑相关结构域, 成环构象, 细胞终末分化

Abstract:

The eukaryotic chromatin is folded into highly complex three-dimensional (3D) structures, which plays an important role in the precise regulation of gene expression and normal physiological function. During differentiation and terminal maturation, cells usually undergo dramatic morphology and gene expression changes, accompanied by significant changes in the 3D structure of the genome. In this review, we provide a comprehensive view of the spatial hierarchical organization of the genome, including chromosome territories, A/B compartment, topologically associating domains (TADs) and looping, focusing on recent progresses in the dynamic 3D genomic structural changes and functional regulation during cell differentiation and terminal maturation. In the end, we summarize the unsolved issues as well as prospects of the 3D genome research in cell differentiation and maturation.

Key words: 3D genome, A/B compartment, topologically associating domain (TAD), looping, cell terminal differentiation