遗传 ›› 2021, Vol. 43 ›› Issue (2): 134-141.doi: 10.16288/j.yczz.20-246

• 综述 • 上一篇    

文昌鱼左右体轴建立机制的研究进展

胡广伟1,2(), 张珍珍1,2, 高焕1,2   

  1. 1 江苏海洋大学,江苏省海洋生物资源与环境重点实验室/江苏省海洋生物技术重点实验室,连云港 222005
    2 江苏海洋大学,江苏省海洋生物产业技术协同创新中心,连云港 222005
  • 收稿日期:2020-07-28 发布日期:2020-12-30
  • 基金资助:
    国家自然科学基金项目(31900370);,江苏省自然科学基金项目(BK20191007);江苏省高校优势学科建设工程项目资助

Progress on the left-right asymmetry patterning in amphioxus

Guangwei Hu1,2(), Zhenzhen Zhang1,2, Huan Gao1,2   

  1. 1 Jiangsu Key Laboratory of Marine Bioresources and Environment /Jiangsu Key Laboratory of Marine Biotechnology School of Marine Science and Fisheries, Jiangsu Ocean University, Lianyungang 222005, China
    2 Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang 222005, China
  • Received:2020-07-28 Published:2020-12-30
  • Supported by:
    the National Natural Science Foundation of China(31900370);the Natural Science Foundation of Jiangsu Province(BK20191007);and the Priority Academic Program Development Fund of Jiangsu Higher Education Institutions(PAPD)

摘要:

两侧对称动物左右体轴建立机制研究是发育生物学领域重要的基础科学问题之一。文昌鱼(amphioxus)由于其特殊的进化地位以及与脊椎动物相似的胚胎发育模式和身体构筑方式,是研究动物左右体轴建立机制的理想模式物种。近年来随着文昌鱼室内全人工繁育技术、高效显微注射技术和基因敲除技术的建立,国内外学者在左右体轴建立机制研究上取得了丰硕的成果。本文从文昌鱼胚胎左右不对称发育特点出发,总结了近期文昌鱼左右体轴建立方面取得的研究进展,并提出了文昌鱼左右体轴调控网络图:纤毛运动导致Hh蛋白在文昌鱼中不对称分布(L<R),从而导致Hh信号通路的不对称激活以及Cer的不对称表达(L<R),Cer对Nodal具有抑制作用,由此导致依赖于Nodal活性的基因呈左右不对称表达并最终诱导胚胎左右体轴的正确建立;BMP信号可能并不提供左右不对称信号,但却是CerNodal正确表达的必要条件。

关键词: 文昌鱼, 左右体轴, 胚胎发育

Abstract:

The mechanisms underlying the establishment of left-right (L-R) asymmetry in bilaterians is one of the central enigmas in developmental biology. Amphioxus is an important model in studying the mechanisms of animal asymmetry specification due to its particular phylogenetic position, vertebrate-like embryogenesis and body plan. Recently, with the establishments of artificial breeding technology, high-efficiency microinjection method and gene knockout technology, researchers have successfully dissected the mechanisms of amphioxus L-R asymmetry development. In this review, we summarize the major progress in understanding L-R asymmetry specification in amphioxus and propose a model of regulation of L-R asymmetry in this species. Hh protein is transported dominantly to the right side by cilia movement, leading to R>L Hh signaling andCerexpression. Cer inhibits expression of Nodal, leading to the asymmetric expression of Nodal-dependent genes. The L-R differences in the propagation of the Nodal pathway result in the correct morphological L-R asymmetry development in amphioxus embryo. BMP signaling probably does not provide the asymmetric cue, but is necessary for correct expression ofCer andNodal.

Key words: amphioxus, L-R axis, embryonic development