拟南芥ABI5基因对BR胁迫响应及其对下胚轴生长的调节作用

doi:10.16288/j.yczz.21-199

遗传 ›› 2021, Vol. 43 ›› Issue (9): 901-909.doi: 10.16288/j.yczz.21-199

拟南芥ABI5基因对BR胁迫响应及其对下胚轴生长的调节作用

林建辉¹(), 刘自广², 张楠¹, 严容¹, 张楠¹, 何鑫淼², 王文涛², 刘娣², 吴娟¹()

1. 东北林业大学生命科学学院,东北盐碱植被恢复与重建教育部重点实验室,哈尔滨 150040
2. 黑龙江省农业科学院畜牧研究所,农业农村部种养结合重点实验室,哈尔滨 150028

收稿日期:2021-06-07 修回日期:2021-08-16 出版日期:2021-09-20 发布日期:2021-09-09
通讯作者: 吴娟 E-mail:17862072380@139.com;wuj1970@163.com
作者简介:林建辉,在读硕士研究生,专业方向：生物化学与分子生物学。E-mail: 17862072380@139.com
基金资助:
中央高校基本科研业务费专项资金项目编号(2572020DY09);黑龙江省科学基金项目编号(LH2019C007);财政部和农业农村部：国家现代农业产业技术体系资助

Stress response of ABI5 to BR stress and its regulation on hypocotyls growth in Arabidopsis thaliana

Jianhui Lin¹(), Ziguang Liu², Nan Zhang¹, Rong Yan¹, Nan Zhang¹, Xinmiao He², Wentao Wang², Di Liu², Juan Wu¹()

1. College of Life Sciences, Northeast Forestry University, Key Laboratory of Northeast Salinity and Vegetation Restoration and Reconstruction, Ministry of Education, Harbin 150040, China
2. Institute of Animal Husbandry, Heilongjiang Academy of Agricultural Sciences, Key Laboratory of Combination of Planting and Breeding, Ministry of Agriculture and Rural Affairs, Harbin 150028, China

Received:2021-06-07 Revised:2021-08-16 Online:2021-09-20 Published:2021-09-09
Contact: Wu Juan E-mail:17862072380@139.com;wuj1970@163.com
Supported by:
Supported by the Special Fund for Basic Scientific Research Operation Fees of Central Universities No(2572020DY09);Heilongjiang Science Foundation Project No(LH2019C007);China Agriculture Research System of MOF and MARA

摘要/Abstract

摘要：

植物脱落酸不敏感蛋白5 (abscisic acid-insensitive 5, ABI5)是种子中大量表达的碱性亮氨酸拉链类型(basic leucine zipper, bZIP)转录因子,在调节种子萌发和幼苗早期生长的脱落酸(abscisic acid, ABA)信号中起着核心作用。油菜素内酯(brassinosteroid, BR)是一种新型植物内源激素,具有调节植物生长发育和逆境胁迫响应等诸多生理功能。近期研究发现,油菜素内酯胁迫条件下,BR信号通路中BIN2 (BRASSINOSTEROID INSENSITIVE2)和BES1 (BRI1-EMS-SUPPRESSOR 1)通过抑制ABI5表达,促进拟南芥(Arabidopsis thaliana)种子萌发。为进一步探究BR胁迫下ABI5功能,本研究分析了种子萌发期ABI5表达特性,鉴定出拟南芥ABI5基因缺失突变体abi5-1并对BR胁迫下其功能进行解析。结果表明：ABI5在拟南芥干种子中大量表达并响应萌发期BR胁迫;正常条件下,abi5-1与野生型幼苗下胚轴无明显差异;BR胁迫下,abi5-1幼苗下胚轴明显长于野生型。本研究结果揭示了ABI5调控BR胁迫下拟南芥下胚轴生长,为深入了解ABI5调节植物发育的分子机制提供了依据。

关键词: ABI5, 油菜素内酯, 拟南芥, 下胚轴

Abstract:

Abscisic acid-insensitive 5 (ABI5) is a basic leucine zipper (bZIP) transcription factor that is abundantly expressed in seeds. It plays a central role in regulating the abscisic acid (ABA) signal of seed germination and early seedling growth. Brassinosteroid (BR) is a new type of plant endogenous hormone, which has many physiological functions such as regulating plant growth and development and response to adversity stress. It has recently been discovered that under brassinolide stress, BIN2 (BRASSINOSTEROID INSENSITIVE2) and BES1 (BRI1-EMS-SUPPRESSOR 1) in the BR signaling pathway can inhibit the expression of ABI5 and promote Arabidopsis thaliana seed germination. In order to further explore the function of ABI5 under BR stress, this study analyzed the ABI5 expression characteristics during seed germination, identified Arabidopsis ABI5 gene deletion mutant abi5-1 and analyzed its function under BR stress, the results of which indicated that ABI5 was abundantly expressed in Arabidopsis dry seeds and responded to BR stress during germination. Under normal conditions, there was no significant difference between the hypocotyls of abi5-1 and wild-type seedlings; but under BR stress, the hypocotyls of abi5-1 seedlings were significantly longer than those of wild-type seedlings. These results reveal that ABI5 regulates the growth of Arabidopsis hypocotyls under BR stress, thereby providing a basis for in-depth understanding of the molecular mechanism of ABI5 regulation on plant development.

Key words: ABI5, brassinosteroid, Arabidopsis thaliana, hypocotyl

林建辉, 刘自广, 张楠, 严容, 张楠, 何鑫淼, 王文涛, 刘娣, 吴娟. 拟南芥ABI5基因对BR胁迫响应及其对下胚轴生长的调节作用[J]. 遗传, 2021, 43(9): 901-909.

Jianhui Lin, Ziguang Liu, Nan Zhang, Rong Yan, Nan Zhang, Xinmiao He, Wentao Wang, Di Liu, Juan Wu. Stress response of ABI5 to BR stress and its regulation on hypocotyls growth in Arabidopsis thaliana[J]. Hereditas(Beijing), 2021, 43(9): 901-909.

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引物名称	引物序列(5ʹ→3ʹ)	复性温度(℃)	用途
Actin2-F	GGTAACATTGTGCTCAGTGGTGG	58	qRT-PCR
Actin2-R	AACGACCTTAATCTTCATGCTGC	58	qRT-PCR
abi5-1-F	CGAGGGTGGTGTTGGTGTCTTTA	60	突变体鉴定
abi5-1-R	CTACTCCATACTGACCTCCTA	60	突变体鉴定
ABI5-F(q)	AACCTAATCCAACCCGAACC	60	qRT-PCR
ABI5-R(q)	ACCCTCCTCCTCCTGTCC	60	qRT-PCR
BIN2-F(q)	ACAAAAGGATGCCCCCAGAA	59	qRT-PCR
BIN2-R(q)	TGAAGTTGAAGAGAGGCGGG	59	qRT-PCR
BES1-F(q)	GCAATTGTCTCCAAACACAGCAG	61	qRT-PCR
BES1-R(q)	CTCCAATCCTTCCTTCCGACATG	61	qRT-PCR
BZR1-F(q)	GCAGATGTCTCCAAATACTGCTG	61	qRT-PCR
BZR1-R(q)	GACATGCCATTTGGGTTTGCCTAG	61	qRT-PCR
PIN7-F(q)	GTGGGATGTGGCAATGCCTAA	60	qRT-PCR
PIN7-R(q)	TCCAATAGCCATTGCTGCCAC	60	qRT-PCR
EXP3-F(q)	GGAACTTGTACAGCCAAGGATA	57	qRT-PCR
EXP3-R(q)	AATAGATGGATTTCCCGGAACA	57	qRT-PCR

拟南芥ABI5基因对BR胁迫响应及其对下胚轴生长的调节作用

Stress response of ABI5 to BR stress and its regulation on hypocotyls growth in Arabidopsis thaliana

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