fC31整合酶系统介导的位点特异性整合研究进展

doi:10.3724/SP.J.1005.2011.00567

遗传 ›› 2011, Vol. 33 ›› Issue (6): 567-575.doi: 10.3724/SP.J.1005.2011.00567

fC31整合酶系统介导的位点特异性整合研究进展

马晴雯

上海交通大学附属儿童医院, 上海交通大学医学遗传研究所, 上海 200040

收稿日期:2011-01-17 修回日期:2011-04-06 出版日期:2011-06-20 发布日期:2011-06-25
通讯作者: 马晴雯 E-mail:maqingwen@hotmail.com
基金资助:
转基因生物新品种培育科技重大专项(编号：2009ZX08010-018B)

Progress of fC31 integrase system in site-specific integration

MA Qing-Wen

Children’s Hospital of Shanghai, Institute of Medical Genetics, Shanghai JiaoTong University, Shanghai 200040, China

Received:2011-01-17 Revised:2011-04-06 Online:2011-06-20 Published:2011-06-25
Contact: MA Qing-Wen E-mail:maqingwen@hotmail.com

摘要/Abstract

摘要： 来源于链霉菌(Streptomyces)噬菌体fC31的整合酶可介导链霉菌附着位点(attB)和噬菌体附着位点(attP)之间的同源重组, 这种重组亦可在多种动植物细胞内进行; 而且, 该整合酶还可介导含attB位点的载体以位点特异性方式整合于多种真核生物基因组内的假attP位点, 并使转基因持续高效表达。因此, fC31整合酶在基因修饰、基因治疗及转基因动物研制等方面得到了广泛的应用。文章就近年来fC31整合酶整合规律、提高效率方面的改进及安全性等相关领域的研究进展进行了综述。

关键词: fC31整合酶, 转基因, 安全性, 位点特异性整合, 基因治疗

Abstract: Integrase of phage fC31 catalyses the homologous recombination between Streptomyces attachment site attB and the phage attachment site attP. Meanwhile, this integrase can mediate integration of attB-containing donor plasmids into the pseudo attP sites in eukaryotic genomes by a site-specific manner and resulting long-term and robust expression of integrated genes. Nowadays, fC31 integrase system is becoming a potential tool for genome modification, gene therapy and transgenic research. Recent progress of fC31 integrase system in integration mode in mammalian genomes, efficiency improvement and researches concerned on transgenic safety were summarized in this review.

Key words: site-specific integration, gene therapy, transgenic, safety, fC31 integrase

马晴雯. fC31整合酶系统介导的位点特异性整合研究进展[J]. 遗传, 2011, 33(6): 567-575.

MA Qing-Wen. Progress of fC31 integrase system in site-specific integration[J]. HEREDITAS, 2011, 33(6): 567-575.

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fC31整合酶系统介导的位点特异性整合研究进展

Progress of fC31 integrase system in site-specific integration

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